Schäfer, Benedikt ; Emonts, Caroline ; Glimpel, Nikola ; Ruhl, Tim ; Obrecht, Astrid S. ; Jockenhoevel, Stefan ; Gries, Thomas ; Beier, Justus P. ; Blaeser, Andreas (2020)
Warp-knitted spacer fabrics : a versatile platform to generate fiber-reinforced hydrogels for 3D tissue engineering.
In: Materials, 13 (16)
doi: 10.3390/ma13163518
Artikel, Bibliographie
Dies ist die neueste Version dieses Eintrags.
Kurzbeschreibung (Abstract)
Mesenchymal stem cells (MSCs) possess huge potential for regenerative medicine. For tissue engineering approaches, scaffolds and hydrogels are routinely used as extracellular matrix (ECM) carriers. The present study investigated the feasibility of using textile-reinforced hydrogels with adjustable porosity and elasticity as a versatile platform for soft tissue engineering. A warp-knitted poly (ethylene terephthalate) (PET) scaffold was developed and characterized with respect to morphology, porosity, and mechanics. The textile carrier was infiltrated with hydrogels and cells resulting in a fiber-reinforced matrix with adjustable biological as well as mechanical cues. Finally, the potential of this platform technology for regenerative medicine was tested on the example of fat tissue engineering. MSCs were seeded on the construct and exposed to adipogenic differentiation medium. Cell invasion was detected by two-photon microscopy, proliferation was measured by the PrestoBlue assay. Successful adipogenesis was demonstrated using Oil Red O staining as well as measurement of secreted adipokines. In conclusion, the given microenvironment featured optimal mechanical as well as biological properties for proliferation and differentiation of MSCs. Besides fat tissue, the textile-reinforced hydrogel system with adjustable mechanics could be a promising platform for future fabrication of versatile soft tissues, such as cartilage, tendon, or muscle.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2020 |
| Autor(en): | Schäfer, Benedikt ; Emonts, Caroline ; Glimpel, Nikola ; Ruhl, Tim ; Obrecht, Astrid S. ; Jockenhoevel, Stefan ; Gries, Thomas ; Beier, Justus P. ; Blaeser, Andreas |
| Art des Eintrags: | Bibliographie |
| Titel: | Warp-knitted spacer fabrics : a versatile platform to generate fiber-reinforced hydrogels for 3D tissue engineering |
| Sprache: | Englisch |
| Publikationsjahr: | 2020 |
| Verlag: | MDPI |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Materials |
| Jahrgang/Volume einer Zeitschrift: | 13 |
| (Heft-)Nummer: | 16 |
| Kollation: | 16 Seiten |
| DOI: | 10.3390/ma13163518 |
| Zugehörige Links: | |
| Kurzbeschreibung (Abstract): | Mesenchymal stem cells (MSCs) possess huge potential for regenerative medicine. For tissue engineering approaches, scaffolds and hydrogels are routinely used as extracellular matrix (ECM) carriers. The present study investigated the feasibility of using textile-reinforced hydrogels with adjustable porosity and elasticity as a versatile platform for soft tissue engineering. A warp-knitted poly (ethylene terephthalate) (PET) scaffold was developed and characterized with respect to morphology, porosity, and mechanics. The textile carrier was infiltrated with hydrogels and cells resulting in a fiber-reinforced matrix with adjustable biological as well as mechanical cues. Finally, the potential of this platform technology for regenerative medicine was tested on the example of fat tissue engineering. MSCs were seeded on the construct and exposed to adipogenic differentiation medium. Cell invasion was detected by two-photon microscopy, proliferation was measured by the PrestoBlue assay. Successful adipogenesis was demonstrated using Oil Red O staining as well as measurement of secreted adipokines. In conclusion, the given microenvironment featured optimal mechanical as well as biological properties for proliferation and differentiation of MSCs. Besides fat tissue, the textile-reinforced hydrogel system with adjustable mechanics could be a promising platform for future fabrication of versatile soft tissues, such as cartilage, tendon, or muscle. |
| Sachgruppe der Dewey Dezimalklassifikatin (DDC): | 600 Technik, Medizin, angewandte Wissenschaften > 600 Technik |
| Fachbereich(e)/-gebiet(e): | 16 Fachbereich Maschinenbau 16 Fachbereich Maschinenbau > Institut für Druckmaschinen und Druckverfahren (IDD) |
| Hinterlegungsdatum: | 02 Aug 2024 12:35 |
| Letzte Änderung: | 02 Aug 2024 12:35 |
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| Suche nach Titel in: | TUfind oder in Google |
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Warp-knitted spacer fabrics : a versatile platform to generate fiber-reinforced hydrogels for 3D tissue engineering. (deposited 23 Mär 2021 08:11)
- Warp-knitted spacer fabrics : a versatile platform to generate fiber-reinforced hydrogels for 3D tissue engineering. (deposited 02 Aug 2024 12:35) [Gegenwärtig angezeigt]
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