Joseph, Franziska M. ; Kaldenhoff, Ralf (2024)
Tobacco aquaporin NtAQP1 and human aquaporin hAQP1 contribute to single cell photosynthesis in Synechococcus.
In: Biology of the cell
doi: 10.1111/boc.202470003
Artikel, Bibliographie
Kurzbeschreibung (Abstract)
BACKGROUND INFORMATION
Aquaporins are HO-permeable membrane protein pores. However, some aquaporins are also permeable to other substances such as CO. In higher plants, overexpression of such aquaporins has already led to an enhanced photosynthetic performance due to improved CO mesophyll conductance. In this work, we investigated the effects of such aquaporins on unicellular photosynthetically active organisms, specifically cyanobacteria.
RESULTS
Overexpression of aquaporins NtAQP1 or hAQP1 that might have a function to improve CO membrane permeability lead to increased photosynthesis rates in the cyanobacterium Synechococcus sp. PCC7002 as concluded by the rate of evolved O. A shift in the Plastoquinone pool state of the cells supports our findings. Water permeable aquaporins without CO permeability, such as NtPIP2;1, do not have this effect.
CONCLUSIONS AND SIGNIFICANCE
We conclude that also in single cell organisms like cyanobacteria, membrane CO conductivity could be rate limiting and CO-porins reduce the respective membrane resistance. We could show that besides the tobacco aquaporin NtAQP1 also the human hAQP1 most likely functions as CO diffusion facilitator in the photosynthesis assay.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2024 |
| Autor(en): | Joseph, Franziska M. ; Kaldenhoff, Ralf |
| Art des Eintrags: | Bibliographie |
| Titel: | Tobacco aquaporin NtAQP1 and human aquaporin hAQP1 contribute to single cell photosynthesis in Synechococcus |
| Sprache: | Englisch |
| Publikationsjahr: | 23 April 2024 |
| Verlag: | Wiley |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Biology of the cell |
| DOI: | 10.1111/boc.202470003 |
| Kurzbeschreibung (Abstract): | BACKGROUND INFORMATION Aquaporins are HO-permeable membrane protein pores. However, some aquaporins are also permeable to other substances such as CO. In higher plants, overexpression of such aquaporins has already led to an enhanced photosynthetic performance due to improved CO mesophyll conductance. In this work, we investigated the effects of such aquaporins on unicellular photosynthetically active organisms, specifically cyanobacteria. RESULTS Overexpression of aquaporins NtAQP1 or hAQP1 that might have a function to improve CO membrane permeability lead to increased photosynthesis rates in the cyanobacterium Synechococcus sp. PCC7002 as concluded by the rate of evolved O. A shift in the Plastoquinone pool state of the cells supports our findings. Water permeable aquaporins without CO permeability, such as NtPIP2;1, do not have this effect. CONCLUSIONS AND SIGNIFICANCE We conclude that also in single cell organisms like cyanobacteria, membrane CO conductivity could be rate limiting and CO-porins reduce the respective membrane resistance. We could show that besides the tobacco aquaporin NtAQP1 also the human hAQP1 most likely functions as CO diffusion facilitator in the photosynthesis assay. |
| ID-Nummer: | pmid:38653736 |
| Zusätzliche Informationen: | Artikel-ID: e2470003 |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie 10 Fachbereich Biologie > Applied Plant Sciences |
| Hinterlegungsdatum: | 29 Apr 2024 11:20 |
| Letzte Änderung: | 29 Apr 2024 11:25 |
| PPN: | 517624095 |
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