Blohm, Annika ; Kumar, Swatantar ; Knebl, Andreas ; Herrmann, Martina ; Küsel, Kirsten ; Popp, Jürgen ; Frosch, Torsten (2024)
Activity and electron donor preference of two denitrifying bacterial strains identified by Raman gas spectroscopy.
In: Analytical and Bioanalytical Chemistry, 2022, 414 (1)
doi: 10.26083/tuprints-00023436
Artikel, Zweitveröffentlichung, Verlagsversion
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Kurzbeschreibung (Abstract)
Human activities have greatly increased the input of reactive nitrogen species into the environment and disturbed the balance of the global N cycle. This imbalance may be offset by bacterial denitrification, an important process in maintaining the ecological balance of nitrogen. However, our understanding of the activity of mixotrophic denitrifying bacteria is not complete, as most research has focused on heterotrophic denitrification. The aim of this study was to investigate substrate preferences for two mixotrophic denitrifying bacterial strains, Acidovorax delafieldii and Hydrogenophaga taeniospiralis, under heterotrophic, autotrophic or mixotrophic conditions. This complex analysis was achieved by simultaneous identification and quantification of H₂, O₂, CO₂, ¹⁴N₂, ¹⁵N₂ and ¹⁵N₂O in course of the denitrification process with help of cavity-enhanced Raman spectroscopic (CERS) multi-gas analysis. To disentangle electron donor preferences for both bacterial strains, microcosm-based incubation experiments under varying substrate conditions were conducted. We found that Acidovorax delafieldii preferentially performed heterotrophic denitrification in the mixotrophic sub-experiments, while Hydrogenophaga taeniospiralis preferred autotrophic denitrification in the mixotrophic incubation. These observations were supported by stoichiometric calculations. The results demonstrate the prowess of advanced Raman multi-gas analysis to study substrate use and electron donor preferences in denitrification, based on the comprehensive quantification of complex microbial gas exchange processes.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2024 |
| Autor(en): | Blohm, Annika ; Kumar, Swatantar ; Knebl, Andreas ; Herrmann, Martina ; Küsel, Kirsten ; Popp, Jürgen ; Frosch, Torsten |
| Art des Eintrags: | Zweitveröffentlichung |
| Titel: | Activity and electron donor preference of two denitrifying bacterial strains identified by Raman gas spectroscopy |
| Sprache: | Englisch |
| Publikationsjahr: | 18 März 2024 |
| Ort: | Darmstadt |
| Publikationsdatum der Erstveröffentlichung: | Januar 2022 |
| Ort der Erstveröffentlichung: | Berlin ; Heidelberg |
| Verlag: | Springer |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Analytical and Bioanalytical Chemistry |
| Jahrgang/Volume einer Zeitschrift: | 414 |
| (Heft-)Nummer: | 1 |
| DOI: | 10.26083/tuprints-00023436 |
| URL / URN: | https://tuprints.ulb.tu-darmstadt.de/23436 |
| Zugehörige Links: | |
| Herkunft: | Zweitveröffentlichung DeepGreen |
| Kurzbeschreibung (Abstract): | Human activities have greatly increased the input of reactive nitrogen species into the environment and disturbed the balance of the global N cycle. This imbalance may be offset by bacterial denitrification, an important process in maintaining the ecological balance of nitrogen. However, our understanding of the activity of mixotrophic denitrifying bacteria is not complete, as most research has focused on heterotrophic denitrification. The aim of this study was to investigate substrate preferences for two mixotrophic denitrifying bacterial strains, Acidovorax delafieldii and Hydrogenophaga taeniospiralis, under heterotrophic, autotrophic or mixotrophic conditions. This complex analysis was achieved by simultaneous identification and quantification of H₂, O₂, CO₂, ¹⁴N₂, ¹⁵N₂ and ¹⁵N₂O in course of the denitrification process with help of cavity-enhanced Raman spectroscopic (CERS) multi-gas analysis. To disentangle electron donor preferences for both bacterial strains, microcosm-based incubation experiments under varying substrate conditions were conducted. We found that Acidovorax delafieldii preferentially performed heterotrophic denitrification in the mixotrophic sub-experiments, while Hydrogenophaga taeniospiralis preferred autotrophic denitrification in the mixotrophic incubation. These observations were supported by stoichiometric calculations. The results demonstrate the prowess of advanced Raman multi-gas analysis to study substrate use and electron donor preferences in denitrification, based on the comprehensive quantification of complex microbial gas exchange processes. |
| Freie Schlagworte: | Cavity-enhanced Raman spectroscopy, Denitrification, Multi-gas analysis, Nitrogen cycle |
| Status: | Verlagsversion |
| URN: | urn:nbn:de:tuda-tuprints-234368 |
| Sachgruppe der Dewey Dezimalklassifikatin (DDC): | 500 Naturwissenschaften und Mathematik > 540 Chemie 500 Naturwissenschaften und Mathematik > 570 Biowissenschaften, Biologie 600 Technik, Medizin, angewandte Wissenschaften > 620 Ingenieurwissenschaften und Maschinenbau |
| Fachbereich(e)/-gebiet(e): | 18 Fachbereich Elektrotechnik und Informationstechnik 18 Fachbereich Elektrotechnik und Informationstechnik > Biophotonik-Medizintechnik |
| Hinterlegungsdatum: | 18 Mär 2024 13:34 |
| Letzte Änderung: | 19 Mär 2024 10:46 |
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| Suche nach Titel in: | TUfind oder in Google |
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- Activity and electron donor preference of two denitrifying bacterial strains identified by Raman gas spectroscopy. (deposited 18 Mär 2024 13:34) [Gegenwärtig angezeigt]
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