Mueller, Lena K. ; Baumruck, Andreas C. ; Zhdanova, Hanna ; Tietze, Alesia A. (2020)
Challenges and perspectives in chemical synthesis of highly hydrophobic peptides.
In: Frontiers in Bioengineering and Biotechnology, 8
doi: 10.3389/fbioe.2020.00162
Artikel, Bibliographie
Dies ist die neueste Version dieses Eintrags.
Kurzbeschreibung (Abstract)
Solid phase peptide synthesis (SPPS) provides the possibility to chemically synthesize peptides and proteins. Applying the method on hydrophilic structures is usually without major drawbacks but faces extreme complications when it comes to "difficult sequences." These includes the vitally important, ubiquitously present and structurally demanding membrane proteins and their functional parts, such as ion channels, G-protein receptors, and other pore-forming structures. Standard synthetic and ligation protocols are not enough for a successful synthesis of these challenging sequences. In this review we highlight, summarize and evaluate the possibilities for synthetic production of "difficult sequences" by SPPS, native chemical ligation (NCL) and follow-up protocols.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2020 |
| Autor(en): | Mueller, Lena K. ; Baumruck, Andreas C. ; Zhdanova, Hanna ; Tietze, Alesia A. |
| Art des Eintrags: | Bibliographie |
| Titel: | Challenges and perspectives in chemical synthesis of highly hydrophobic peptides |
| Sprache: | Englisch |
| Publikationsjahr: | 4 März 2020 |
| Ort: | Lausanne |
| Verlag: | Frontiers Media S.A. |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Frontiers in Bioengineering and Biotechnology |
| Jahrgang/Volume einer Zeitschrift: | 8 |
| Kollation: | 17 Seiten |
| DOI: | 10.3389/fbioe.2020.00162 |
| Zugehörige Links: | |
| Kurzbeschreibung (Abstract): | Solid phase peptide synthesis (SPPS) provides the possibility to chemically synthesize peptides and proteins. Applying the method on hydrophilic structures is usually without major drawbacks but faces extreme complications when it comes to "difficult sequences." These includes the vitally important, ubiquitously present and structurally demanding membrane proteins and their functional parts, such as ion channels, G-protein receptors, and other pore-forming structures. Standard synthetic and ligation protocols are not enough for a successful synthesis of these challenging sequences. In this review we highlight, summarize and evaluate the possibilities for synthetic production of "difficult sequences" by SPPS, native chemical ligation (NCL) and follow-up protocols. |
| Freie Schlagworte: | solid phase peptide synthesis, membrane-associated proteins, native chemical ligation, conjugation, transmembrane peptide |
| ID-Nummer: | Artikel-ID: 162 |
| Zusätzliche Informationen: | This article is part of the Research Topic: Engineering of Biological Membranes Specialty section: This article was submitted to Synthetic Biology, a section of the journal Frontiers in Bioengineering and Biotechnology |
| Sachgruppe der Dewey Dezimalklassifikatin (DDC): | 500 Naturwissenschaften und Mathematik > 540 Chemie 500 Naturwissenschaften und Mathematik > 570 Biowissenschaften, Biologie |
| Fachbereich(e)/-gebiet(e): | 07 Fachbereich Chemie 07 Fachbereich Chemie > Clemens-Schöpf-Institut > Fachgebiet Biochemie 07 Fachbereich Chemie > Clemens-Schöpf-Institut |
| Hinterlegungsdatum: | 11 Mär 2024 06:17 |
| Letzte Änderung: | 11 Mär 2024 06:17 |
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| Suche nach Titel in: | TUfind oder in Google |
Verfügbare Versionen dieses Eintrags
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Challenges and Perspectives in Chemical Synthesis of Highly Hydrophobic Peptides. (deposited 08 Mär 2024 13:24)
- Challenges and perspectives in chemical synthesis of highly hydrophobic peptides. (deposited 11 Mär 2024 06:17) [Gegenwärtig angezeigt]
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