Böhm, Alexander ; Trosien, Simon ; Avrutina, Olga ; Kolmar, Harald ; Biesalski, Markus (2024)
Covalent Attachment of Enzymes to Paper Fibers for Paper-Based Analytical Devices.
In: Frontiers in Chemistry, 2018, 6
doi: 10.26083/tuprints-00015860
Artikel, Zweitveröffentlichung, Verlagsversion
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Kurzbeschreibung (Abstract)
Due to its unique material properties, paper offers many practical advantages as a viable platform for sensing devices. In view of paper-based microfluidic biosensing applications, the covalent immobilization of enzymes with preserved functional activity is highly desirable and ultimately challenging. In the present manuscript, we report an efficient approach to achieving the covalent attachment of certain enzymes on paper fibers via a surface-bound network of hydrophilic polymers bearing protein-modifiable sites. This tailor-made macromolecular system consisting of polar, highly swellable copolymers is anchored to the paper exterior upon light-induced crosslinking of engineered benzophenone motifs. On the other hand, this framework contains active esters that can be efficiently modified by the nucleophiles of biomolecules. This strategy allowed the covalent immobilization of glucose oxidase and horseradish peroxidase onto cotton linters without sacrificing their bioactivities and performance upon surface binding. As a proof-of-concept application, a microfluidic chromatic paper-based glucose sensor was developed and achieved successful glucose detection in a simple yet efficient cascade reaction.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2024 |
| Autor(en): | Böhm, Alexander ; Trosien, Simon ; Avrutina, Olga ; Kolmar, Harald ; Biesalski, Markus |
| Art des Eintrags: | Zweitveröffentlichung |
| Titel: | Covalent Attachment of Enzymes to Paper Fibers for Paper-Based Analytical Devices |
| Sprache: | Englisch |
| Publikationsjahr: | 5 März 2024 |
| Ort: | Darmstadt |
| Publikationsdatum der Erstveröffentlichung: | 27 Juni 2018 |
| Ort der Erstveröffentlichung: | Lausanne |
| Verlag: | Frontiers Media S.A. |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Frontiers in Chemistry |
| Jahrgang/Volume einer Zeitschrift: | 6 |
| Kollation: | 10 Seiten |
| DOI: | 10.26083/tuprints-00015860 |
| URL / URN: | https://tuprints.ulb.tu-darmstadt.de/15860 |
| Zugehörige Links: | |
| Herkunft: | Zweitveröffentlichung DeepGreen |
| Kurzbeschreibung (Abstract): | Due to its unique material properties, paper offers many practical advantages as a viable platform for sensing devices. In view of paper-based microfluidic biosensing applications, the covalent immobilization of enzymes with preserved functional activity is highly desirable and ultimately challenging. In the present manuscript, we report an efficient approach to achieving the covalent attachment of certain enzymes on paper fibers via a surface-bound network of hydrophilic polymers bearing protein-modifiable sites. This tailor-made macromolecular system consisting of polar, highly swellable copolymers is anchored to the paper exterior upon light-induced crosslinking of engineered benzophenone motifs. On the other hand, this framework contains active esters that can be efficiently modified by the nucleophiles of biomolecules. This strategy allowed the covalent immobilization of glucose oxidase and horseradish peroxidase onto cotton linters without sacrificing their bioactivities and performance upon surface binding. As a proof-of-concept application, a microfluidic chromatic paper-based glucose sensor was developed and achieved successful glucose detection in a simple yet efficient cascade reaction. |
| Freie Schlagworte: | enzyme immobilization, biofunctional paper, lab-on-paper, microfluidics, paper-based diagnostics, point-of-care diagnostics, glucose sensor |
| ID-Nummer: | Artikel-ID: 214 |
| Status: | Verlagsversion |
| URN: | urn:nbn:de:tuda-tuprints-158603 |
| Zusätzliche Informationen: | This article is part of the Research Topic: Sensing MY Biosphere Specialty section: This article was submitted to Chemical Engineering, a section of the journal Frontiers in Chemistry |
| Sachgruppe der Dewey Dezimalklassifikatin (DDC): | 500 Naturwissenschaften und Mathematik > 540 Chemie |
| Fachbereich(e)/-gebiet(e): | 07 Fachbereich Chemie 07 Fachbereich Chemie > Clemens-Schöpf-Institut > Fachgebiet Biochemie 07 Fachbereich Chemie > Clemens-Schöpf-Institut 07 Fachbereich Chemie > Ernst-Berl-Institut 07 Fachbereich Chemie > Ernst-Berl-Institut > Fachgebiet Makromolekulare Chemie 07 Fachbereich Chemie > Ernst-Berl-Institut > Fachgebiet Makromolekulare Chemie > Makromolekulare Chemie und Papierchemie |
| Hinterlegungsdatum: | 05 Mär 2024 13:43 |
| Letzte Änderung: | 06 Mär 2024 09:13 |
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| Suche nach Titel in: | TUfind oder in Google |
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- Covalent Attachment of Enzymes to Paper Fibers for Paper-Based Analytical Devices. (deposited 05 Mär 2024 13:43) [Gegenwärtig angezeigt]
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