Wolf, Sebastian ; Domes, Robert ; Domes, Christian ; Frosch, Torsten (2024)
Spectrally Resolved and Highly Parallelized Raman Difference Spectroscopy for the Analysis of Drug-Target Interactions between the Antimalarial Drug Chloroquine and Hematin.
In: Analytical Chemistry, 96 (8)
doi: 10.1021/acs.analchem.3c04231
Artikel, Bibliographie
Kurzbeschreibung (Abstract)
Malaria is a severe disease caused by cytozoic parasites of the genus Plasmodium, which infiltrate and infect red blood cells. Several drugs have been developed to combat the devastating effects of malaria. Antimalarials based on quinolines inhibit the crystallization of hematin into hemozoin within the parasite, ultimately leading to its demise. Despite the frequent use of these agents, there are unanswered questions about their mechanisms of action. In the present study, the quinoline chloroquine and its interaction with the target structure hematin was investigated using an advanced, highly parallelized Raman difference spectroscopy (RDS) setup. Simultaneous recording of the spectra of hematin and chloroquine mixtures with varying compositions enabled the observation of changes in peak heights and positions based on the altered molecular structure resulting from their interaction. A shift of (−1.12 ± 0.05) cm–1 was observed in the core-size marker band ν(CαCm)asym peak position of the 1:1 chloroquine–hematin mixture compared to pure hematin. The oxidation-state marker band ν(pyrrole half-ring)sym exhibited a shift by (+0.93 ± 0.13) cm–1. These results were supported by density functional theory (DFT) calculations, indicating a hydrogen bond between the quinolinyl moiety of chloroquine and the oxygen atom of ferric protoporphyrin IX hydroxide (Fe(III)PPIX-OH). The consequence is a reduced electron density within the porphyrin moiety and an increase in its core size. This hypothesis provided further insights into the mechanism of hemozoin inhibition, suggesting chloroquine binding to the monomeric form of hematin, thereby preventing its further crystallization to hemozoin.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2024 |
| Autor(en): | Wolf, Sebastian ; Domes, Robert ; Domes, Christian ; Frosch, Torsten |
| Art des Eintrags: | Bibliographie |
| Titel: | Spectrally Resolved and Highly Parallelized Raman Difference Spectroscopy for the Analysis of Drug-Target Interactions between the Antimalarial Drug Chloroquine and Hematin |
| Sprache: | Englisch |
| Publikationsjahr: | 27 Februar 2024 |
| Verlag: | ACS Publications |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Analytical Chemistry |
| Jahrgang/Volume einer Zeitschrift: | 96 |
| (Heft-)Nummer: | 8 |
| DOI: | 10.1021/acs.analchem.3c04231 |
| Kurzbeschreibung (Abstract): | Malaria is a severe disease caused by cytozoic parasites of the genus Plasmodium, which infiltrate and infect red blood cells. Several drugs have been developed to combat the devastating effects of malaria. Antimalarials based on quinolines inhibit the crystallization of hematin into hemozoin within the parasite, ultimately leading to its demise. Despite the frequent use of these agents, there are unanswered questions about their mechanisms of action. In the present study, the quinoline chloroquine and its interaction with the target structure hematin was investigated using an advanced, highly parallelized Raman difference spectroscopy (RDS) setup. Simultaneous recording of the spectra of hematin and chloroquine mixtures with varying compositions enabled the observation of changes in peak heights and positions based on the altered molecular structure resulting from their interaction. A shift of (−1.12 ± 0.05) cm–1 was observed in the core-size marker band ν(CαCm)asym peak position of the 1:1 chloroquine–hematin mixture compared to pure hematin. The oxidation-state marker band ν(pyrrole half-ring)sym exhibited a shift by (+0.93 ± 0.13) cm–1. These results were supported by density functional theory (DFT) calculations, indicating a hydrogen bond between the quinolinyl moiety of chloroquine and the oxygen atom of ferric protoporphyrin IX hydroxide (Fe(III)PPIX-OH). The consequence is a reduced electron density within the porphyrin moiety and an increase in its core size. This hypothesis provided further insights into the mechanism of hemozoin inhibition, suggesting chloroquine binding to the monomeric form of hematin, thereby preventing its further crystallization to hemozoin. |
| Freie Schlagworte: | Multifocal, RDS, Drug Target Interaction, Antimalarial Drug |
| Zusätzliche Informationen: | Highlighted as journal cover |
| Fachbereich(e)/-gebiet(e): | 18 Fachbereich Elektrotechnik und Informationstechnik 18 Fachbereich Elektrotechnik und Informationstechnik > Biophotonik-Medizintechnik Forschungsfelder Forschungsfelder > Matter and Materials |
| Hinterlegungsdatum: | 20 Mär 2024 14:07 |
| Letzte Änderung: | 06 Jun 2024 12:55 |
| PPN: | 518876209 |
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