Liu, Hui‐Yu ; Kumar, Ravi ; Zhong, Chunting ; Gorji, Saleh ; Paniushkina, Liliia ; Masood, Ramsha ; Wittel, Uwe A. ; Fuchs, Harald ; Nazarenko, Irina ; Hirtz, Michael (2021)
Rapid capture of cancer extracellular vesicles by lipid patch microarrays.
In: Advanced Materials, 33 (35)
doi: 10.1002/adma.202008493
Artikel, Bibliographie
Dies ist die neueste Version dieses Eintrags.
Kurzbeschreibung (Abstract)
Extracellular vesicles (EVs) contain various bioactive molecules such as DNA, RNA, and proteins, and play a key role in the regulation of cancer progression. Furthermore, cancer‐associated EVs carry specific biomarkers and can be used in liquid biopsy for cancer detection. However, it is still technically challenging and time consuming to detect or isolate cancer‐associated EVs from complex biofluids (e.g., blood). Here, a novel EV‐capture strategy based on dip‐pen nanolithography generated microarrays of supported lipid membranes is presented. These arrays carry specific antibodies recognizing EV‐ and cancer‐specific surface biomarkers, enabling highly selective and efficient capture. Importantly, it is shown that the nucleic acid cargo of captured EVs is retained on the lipid array, providing the potential for downstream analysis. Finally, the feasibility of EV capture from patient sera is demonstrated. The demonstrated platform offers rapid capture, high specificity, and sensitivity, with only a small need in analyte volume and without additional purification steps. The platform is applied in context of cancer‐associated EVs, but it can easily be adapted to other diagnostic EV targets by use of corresponding antibodies.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2021 |
| Autor(en): | Liu, Hui‐Yu ; Kumar, Ravi ; Zhong, Chunting ; Gorji, Saleh ; Paniushkina, Liliia ; Masood, Ramsha ; Wittel, Uwe A. ; Fuchs, Harald ; Nazarenko, Irina ; Hirtz, Michael |
| Art des Eintrags: | Bibliographie |
| Titel: | Rapid capture of cancer extracellular vesicles by lipid patch microarrays |
| Sprache: | Englisch |
| Publikationsjahr: | 2021 |
| Ort: | Weinheim |
| Verlag: | Wiley-VCH |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Advanced Materials |
| Jahrgang/Volume einer Zeitschrift: | 33 |
| (Heft-)Nummer: | 35 |
| Kollation: | 13 Seiten |
| DOI: | 10.1002/adma.202008493 |
| Zugehörige Links: | |
| Kurzbeschreibung (Abstract): | Extracellular vesicles (EVs) contain various bioactive molecules such as DNA, RNA, and proteins, and play a key role in the regulation of cancer progression. Furthermore, cancer‐associated EVs carry specific biomarkers and can be used in liquid biopsy for cancer detection. However, it is still technically challenging and time consuming to detect or isolate cancer‐associated EVs from complex biofluids (e.g., blood). Here, a novel EV‐capture strategy based on dip‐pen nanolithography generated microarrays of supported lipid membranes is presented. These arrays carry specific antibodies recognizing EV‐ and cancer‐specific surface biomarkers, enabling highly selective and efficient capture. Importantly, it is shown that the nucleic acid cargo of captured EVs is retained on the lipid array, providing the potential for downstream analysis. Finally, the feasibility of EV capture from patient sera is demonstrated. The demonstrated platform offers rapid capture, high specificity, and sensitivity, with only a small need in analyte volume and without additional purification steps. The platform is applied in context of cancer‐associated EVs, but it can easily be adapted to other diagnostic EV targets by use of corresponding antibodies. |
| Freie Schlagworte: | breast cancer, dip‐pen nanolithography, extracellular vesicles, scanning probe lithography, supported lipid bilayers |
| ID-Nummer: | Artikel-ID: 2008493 |
| Sachgruppe der Dewey Dezimalklassifikatin (DDC): | 600 Technik, Medizin, angewandte Wissenschaften > 600 Technik 600 Technik, Medizin, angewandte Wissenschaften > 610 Medizin, Gesundheit 600 Technik, Medizin, angewandte Wissenschaften > 660 Technische Chemie |
| Fachbereich(e)/-gebiet(e): | 11 Fachbereich Material- und Geowissenschaften 11 Fachbereich Material- und Geowissenschaften > Materialwissenschaft 11 Fachbereich Material- und Geowissenschaften > Materialwissenschaft > Gemeinschaftslabor Nanomaterialien |
| Hinterlegungsdatum: | 14 Feb 2024 06:31 |
| Letzte Änderung: | 14 Feb 2024 06:31 |
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| Suche nach Titel in: | TUfind oder in Google |
Verfügbare Versionen dieses Eintrags
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Rapid Capture of Cancer Extracellular Vesicles by Lipid Patch Microarrays. (deposited 13 Feb 2024 13:44)
- Rapid capture of cancer extracellular vesicles by lipid patch microarrays. (deposited 14 Feb 2024 06:31) [Gegenwärtig angezeigt]
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