Kramat, Janice ; Kraus, Leon ; Gunawan, Vincent J. ; Smyej, Elias ; Froehlich, Philipp ; Weber, Tim E. ; Spiehl, Dieter ; Koeppl, Heinz ; Blaeser, Andreas ; Suess, Beatrix (2024)
Sensing levofloxacin with an RNA aptamer as a bioreceptor.
In: Biosensors, 14 (1)
doi: 10.3390/bios14010056
Artikel, Bibliographie
Dies ist die neueste Version dieses Eintrags.
Kurzbeschreibung (Abstract)
To combat the growing threat of antibiotic resistance, environmental testing for antibiotic contamination is gaining an increasing role. This study aims to develop an easy-to-use assay for the detection of the fluoroquinolone antibiotic levofloxacin. Levofloxacin is used in human and veterinary medicine and has been detected in wastewater and river water. An RNA aptamer against levofloxacin was selected using RNA Capture-SELEX. The 73 nt long aptamer folds into three stems with a central three-way junction. It binds levofloxacin with a of 6 µM and discriminates the closely related compound ciprofloxacin. Furthermore, the selection process was analyzed using a next-generation sequencing approach to better understand the sequence evolution throughout the selection. The aptamer was used as a bioreceptor for the development of a lateral flow assay. The biosensor exploited the innate characteristic of RNA Capture-SELEX to select aptamers that displace a complementary DNA oligonucleotide upon ligand binding. The lateral flow assay achieved a limit of visual detection of 100 µM. While the sensitivity of this assay constrains its immediate use in environmental testing, the present study can serve as a template for the selection of RNA aptamer-based biosensors.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2024 |
| Autor(en): | Kramat, Janice ; Kraus, Leon ; Gunawan, Vincent J. ; Smyej, Elias ; Froehlich, Philipp ; Weber, Tim E. ; Spiehl, Dieter ; Koeppl, Heinz ; Blaeser, Andreas ; Suess, Beatrix |
| Art des Eintrags: | Bibliographie |
| Titel: | Sensing levofloxacin with an RNA aptamer as a bioreceptor |
| Sprache: | Englisch |
| Publikationsjahr: | 22 Januar 2024 |
| Ort: | Basel |
| Verlag: | MDPI |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Biosensors |
| Jahrgang/Volume einer Zeitschrift: | 14 |
| (Heft-)Nummer: | 1 |
| DOI: | 10.3390/bios14010056 |
| Zugehörige Links: | |
| Kurzbeschreibung (Abstract): | To combat the growing threat of antibiotic resistance, environmental testing for antibiotic contamination is gaining an increasing role. This study aims to develop an easy-to-use assay for the detection of the fluoroquinolone antibiotic levofloxacin. Levofloxacin is used in human and veterinary medicine and has been detected in wastewater and river water. An RNA aptamer against levofloxacin was selected using RNA Capture-SELEX. The 73 nt long aptamer folds into three stems with a central three-way junction. It binds levofloxacin with a of 6 µM and discriminates the closely related compound ciprofloxacin. Furthermore, the selection process was analyzed using a next-generation sequencing approach to better understand the sequence evolution throughout the selection. The aptamer was used as a bioreceptor for the development of a lateral flow assay. The biosensor exploited the innate characteristic of RNA Capture-SELEX to select aptamers that displace a complementary DNA oligonucleotide upon ligand binding. The lateral flow assay achieved a limit of visual detection of 100 µM. While the sensitivity of this assay constrains its immediate use in environmental testing, the present study can serve as a template for the selection of RNA aptamer-based biosensors. |
| ID-Nummer: | Artikel-ID: 56 |
| Zusätzliche Informationen: | pmid:38275309 |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie 10 Fachbereich Biologie > Synthetic RNA biology 18 Fachbereich Elektrotechnik und Informationstechnik 18 Fachbereich Elektrotechnik und Informationstechnik > Self-Organizing Systems Lab Interdisziplinäre Forschungsprojekte Interdisziplinäre Forschungsprojekte > Centre for Synthetic Biology |
| Hinterlegungsdatum: | 29 Jan 2024 12:32 |
| Letzte Änderung: | 08 Mai 2024 07:27 |
| PPN: | 515113859 |
| Export: | |
| Suche nach Titel in: | TUfind oder in Google |
Verfügbare Versionen dieses Eintrags
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Sensing Levofloxacin with an RNA Aptamer as a Bioreceptor. (deposited 07 Mai 2024 12:46)
- Sensing levofloxacin with an RNA aptamer as a bioreceptor. (deposited 29 Jan 2024 12:32) [Gegenwärtig angezeigt]
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