Hetzke, Thilo ; Vogel, Marc ; Halbritter, Anna-Lena Johanna ; Saha, Subham ; Suess, Beatrix ; Sigurdsson, Snorri Th. ; Prisner, Thomas F. (2023)
Simultaneous localization of two high affinity divalent metal ion binding sites in the tetracycline RNA aptamer with Mn2+-based pulsed dipolar EPR spectroscopy.
In: The journal of physical chemistry letters, 14 (50)
doi: 10.1021/acs.jpclett.3c02566
Artikel, Bibliographie
Kurzbeschreibung (Abstract)
Mg ions play an essential part in stabilizing the tertiary structure of nucleic acids. While the importance of these ions is well documented, their localization and elucidation of their role in the structure and dynamics of nucleic acids are often challenging. In this work, pulsed electron-electron double resonance spectroscopy (PELDOR, also known as DEER) was used to localize two high affinity divalent metal ion binding sites in the tetracycline RNA aptamer with high accuracy. For this purpose, the aptamer was labeled at different positions with a semirigid nitroxide spin label and diamagnetic Mg was replaced with paramagnetic Mn, which did not alter the folding process or ligand binding. Out of the several divalent metal ion binding sites that are known from the crystal structure, two binding sites with high affinity were detected: one that is located at the ligand binding center and another at the J1/2 junction of the RNA.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2023 |
| Autor(en): | Hetzke, Thilo ; Vogel, Marc ; Halbritter, Anna-Lena Johanna ; Saha, Subham ; Suess, Beatrix ; Sigurdsson, Snorri Th. ; Prisner, Thomas F. |
| Art des Eintrags: | Bibliographie |
| Titel: | Simultaneous localization of two high affinity divalent metal ion binding sites in the tetracycline RNA aptamer with Mn2+-based pulsed dipolar EPR spectroscopy |
| Sprache: | Englisch |
| Publikationsjahr: | 21 Dezember 2023 |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | The journal of physical chemistry letters |
| Jahrgang/Volume einer Zeitschrift: | 14 |
| (Heft-)Nummer: | 50 |
| DOI: | 10.1021/acs.jpclett.3c02566 |
| Kurzbeschreibung (Abstract): | Mg ions play an essential part in stabilizing the tertiary structure of nucleic acids. While the importance of these ions is well documented, their localization and elucidation of their role in the structure and dynamics of nucleic acids are often challenging. In this work, pulsed electron-electron double resonance spectroscopy (PELDOR, also known as DEER) was used to localize two high affinity divalent metal ion binding sites in the tetracycline RNA aptamer with high accuracy. For this purpose, the aptamer was labeled at different positions with a semirigid nitroxide spin label and diamagnetic Mg was replaced with paramagnetic Mn, which did not alter the folding process or ligand binding. Out of the several divalent metal ion binding sites that are known from the crystal structure, two binding sites with high affinity were detected: one that is located at the ligand binding center and another at the J1/2 junction of the RNA. |
| ID-Nummer: | pmid:38084602 |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie 10 Fachbereich Biologie > Synthetic RNA biology |
| Hinterlegungsdatum: | 28 Dez 2023 09:16 |
| Letzte Änderung: | 29 Jan 2024 07:07 |
| PPN: | 514294752 |
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