Duarte Campos, Daniela F. ; Lindsay, Christopher D. ; Roth, Julien G. ; LeSavage, Bauer L. ; Seymour, Alexis J. ; Krajina, Brad A. ; Ribeiro, Ricardo ; Costa, Pedro F. ; Blaeser, Andreas ; Heilshorn, Sarah C. (2023)
Bioprinting Cell- and Spheroid-Laden Protein-Engineered Hydrogels as Tissue-on-Chip Platforms.
In: Frontiers in Bioengineering and Biotechnology, 2020, 8
doi: 10.26083/tuprints-00016294
Artikel, Zweitveröffentlichung, Verlagsversion
 | Es ist eine neuere Version dieses Eintrags verfügbar. |
Kurzbeschreibung (Abstract)
Human tissues, both in health and disease, are exquisitely organized into complex three-dimensional architectures that inform tissue function. In biomedical research, specifically in drug discovery and personalized medicine, novel human-based three-dimensional (3D) models are needed to provide information with higher predictive value compared to state-of-the-art two-dimensional (2D) preclinical models. However, current in vitro models remain inadequate to recapitulate the complex and heterogenous architectures that underlie biology. Therefore, it would be beneficial to develop novel models that could capture both the 3D heterogeneity of tissue (e.g., through 3D bioprinting) and integrate vascularization that is necessary for tissue viability (e.g., through culture in tissue-on-chips). In this proof-of-concept study, we use elastin-like protein (ELP) engineered hydrogels as bioinks for constructing such tissue models, which can be directly dispensed onto endothelialized on-chip platforms. We show that this bioprinting process is compatible with both single cell suspensions of neural progenitor cells (NPCs) and spheroid aggregates of breast cancer cells. After bioprinting, both cell types remain viable in incubation for up to 14 days. These results demonstrate a first step toward combining ELP engineered hydrogels with 3D bioprinting technologies and on-chip platforms comprising vascular-like channels for establishing functional tissue models.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2023 |
| Autor(en): | Duarte Campos, Daniela F. ; Lindsay, Christopher D. ; Roth, Julien G. ; LeSavage, Bauer L. ; Seymour, Alexis J. ; Krajina, Brad A. ; Ribeiro, Ricardo ; Costa, Pedro F. ; Blaeser, Andreas ; Heilshorn, Sarah C. |
| Art des Eintrags: | Zweitveröffentlichung |
| Titel: | Bioprinting Cell- and Spheroid-Laden Protein-Engineered Hydrogels as Tissue-on-Chip Platforms |
| Sprache: | Englisch |
| Publikationsjahr: | 8 Dezember 2023 |
| Ort: | Darmstadt |
| Publikationsdatum der Erstveröffentlichung: | 2020 |
| Ort der Erstveröffentlichung: | Lausanne |
| Verlag: | Frontiers Media S.A. |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Frontiers in Bioengineering and Biotechnology |
| Jahrgang/Volume einer Zeitschrift: | 8 |
| Kollation: | 13 Seiten |
| DOI: | 10.26083/tuprints-00016294 |
| URL / URN: | https://tuprints.ulb.tu-darmstadt.de/16294 |
| Zugehörige Links: | |
| Herkunft: | Zweitveröffentlichung DeepGreen |
| Kurzbeschreibung (Abstract): | Human tissues, both in health and disease, are exquisitely organized into complex three-dimensional architectures that inform tissue function. In biomedical research, specifically in drug discovery and personalized medicine, novel human-based three-dimensional (3D) models are needed to provide information with higher predictive value compared to state-of-the-art two-dimensional (2D) preclinical models. However, current in vitro models remain inadequate to recapitulate the complex and heterogenous architectures that underlie biology. Therefore, it would be beneficial to develop novel models that could capture both the 3D heterogeneity of tissue (e.g., through 3D bioprinting) and integrate vascularization that is necessary for tissue viability (e.g., through culture in tissue-on-chips). In this proof-of-concept study, we use elastin-like protein (ELP) engineered hydrogels as bioinks for constructing such tissue models, which can be directly dispensed onto endothelialized on-chip platforms. We show that this bioprinting process is compatible with both single cell suspensions of neural progenitor cells (NPCs) and spheroid aggregates of breast cancer cells. After bioprinting, both cell types remain viable in incubation for up to 14 days. These results demonstrate a first step toward combining ELP engineered hydrogels with 3D bioprinting technologies and on-chip platforms comprising vascular-like channels for establishing functional tissue models. |
| Freie Schlagworte: | protein engineered hydrogel, bioink, bioprinting, 3D cell culture, tissue model |
| Status: | Verlagsversion |
| URN: | urn:nbn:de:tuda-tuprints-162945 |
| Zusätzliche Informationen: | This article is part of the Research Topic: 3D Printing for Implantable Medical Devices: From Surgical Reconstruction to Tissue/Organ Regeneration |
| Sachgruppe der Dewey Dezimalklassifikatin (DDC): | 500 Naturwissenschaften und Mathematik > 570 Biowissenschaften, Biologie 600 Technik, Medizin, angewandte Wissenschaften > 610 Medizin, Gesundheit 600 Technik, Medizin, angewandte Wissenschaften > 620 Ingenieurwissenschaften und Maschinenbau |
| Fachbereich(e)/-gebiet(e): | 16 Fachbereich Maschinenbau 16 Fachbereich Maschinenbau > Institut für Druckmaschinen und Druckverfahren (IDD) 16 Fachbereich Maschinenbau > Institut für Druckmaschinen und Druckverfahren (IDD) > Biomedizinische Drucktechnologie (BMT) |
| Hinterlegungsdatum: | 08 Dez 2023 14:51 |
| Letzte Änderung: | 11 Dez 2023 08:00 |
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| Zugehörige Links: | |
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| Suche nach Titel in: | TUfind oder in Google |
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- Bioprinting Cell- and Spheroid-Laden Protein-Engineered Hydrogels as Tissue-on-Chip Platforms. (deposited 08 Dez 2023 14:51) [Gegenwärtig angezeigt]
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