Purr, Foelke ; Eckardt, Max-Frederik ; Kieserling, Jonas ; Gronwald, Paul-Luis ; Burg, Thomas P. ; Dietzel, Andreas (2023)
Robust Smartphone Assisted Biosensing Based on Asymmetric Nanofluidic Grating Interferometry.
In: Sensors, 2019, 19 (9)
doi: 10.26083/tuprints-00015864
Artikel, Zweitveröffentlichung, Verlagsversion
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Kurzbeschreibung (Abstract)
Point-of-care systems enable fast therapy decisions on site without the need of any healthcare infrastructure. In addition to the sensitive detection, stable measurement by inexperienced persons outside of laboratory facilities is indispensable. A particular challenge in field applications is to reduce interference from environmental factors, such as temperature, to acceptable levels without sacrificing simplicity. Here, we present a smartphone-based point-of-care sensor. The method uses an optofluidic grating composed of alternating detection and reference channels arranged as a reflective phase grating. Biomolecules adsorbing to the detection channel alter the optical path length, while the parallel reference channels enable a direct common mode rejection within a single measurement. The optical setup is integrated in a compact design of a mobile readout device and the usability is ensured by a smartphone application. Our results show that different ambient temperatures do not have any influence on the signal. In a proof-of concept experiment we measured the accumulation of specific molecules in functionalized detection channels in real-time and without the need of any labeling. Therefore, the channel walls have been modified with biotin as capture molecules and the specific binding of streptavidin was detected. A mobile, reliable and robust point-of-care device has been realized by combining an inherently differential measurement concept with a smartphone-based, mobile readout device.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2023 |
| Autor(en): | Purr, Foelke ; Eckardt, Max-Frederik ; Kieserling, Jonas ; Gronwald, Paul-Luis ; Burg, Thomas P. ; Dietzel, Andreas |
| Art des Eintrags: | Zweitveröffentlichung |
| Titel: | Robust Smartphone Assisted Biosensing Based on Asymmetric Nanofluidic Grating Interferometry |
| Sprache: | Englisch |
| Publikationsjahr: | 4 Dezember 2023 |
| Ort: | Darmstadt |
| Publikationsdatum der Erstveröffentlichung: | 2019 |
| Ort der Erstveröffentlichung: | Basel |
| Verlag: | MDPI |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Sensors |
| Jahrgang/Volume einer Zeitschrift: | 19 |
| (Heft-)Nummer: | 9 |
| Kollation: | 14 Seiten |
| DOI: | 10.26083/tuprints-00015864 |
| URL / URN: | https://tuprints.ulb.tu-darmstadt.de/15864 |
| Zugehörige Links: | |
| Herkunft: | Zweitveröffentlichung DeepGreen |
| Kurzbeschreibung (Abstract): | Point-of-care systems enable fast therapy decisions on site without the need of any healthcare infrastructure. In addition to the sensitive detection, stable measurement by inexperienced persons outside of laboratory facilities is indispensable. A particular challenge in field applications is to reduce interference from environmental factors, such as temperature, to acceptable levels without sacrificing simplicity. Here, we present a smartphone-based point-of-care sensor. The method uses an optofluidic grating composed of alternating detection and reference channels arranged as a reflective phase grating. Biomolecules adsorbing to the detection channel alter the optical path length, while the parallel reference channels enable a direct common mode rejection within a single measurement. The optical setup is integrated in a compact design of a mobile readout device and the usability is ensured by a smartphone application. Our results show that different ambient temperatures do not have any influence on the signal. In a proof-of concept experiment we measured the accumulation of specific molecules in functionalized detection channels in real-time and without the need of any labeling. Therefore, the channel walls have been modified with biotin as capture molecules and the specific binding of streptavidin was detected. A mobile, reliable and robust point-of-care device has been realized by combining an inherently differential measurement concept with a smartphone-based, mobile readout device. |
| Freie Schlagworte: | biosensing, interferometry, portable point-of-care (POC), common mode rejection, nanofluidic, optofluidic grating, smartphone-based |
| Status: | Verlagsversion |
| URN: | urn:nbn:de:tuda-tuprints-158648 |
| Zusätzliche Informationen: | This article belongs to the Special Issue Portable Biosensing Systems for Point-of-Care Diagnostic Applications |
| Sachgruppe der Dewey Dezimalklassifikatin (DDC): | 600 Technik, Medizin, angewandte Wissenschaften > 621.3 Elektrotechnik, Elektronik |
| Fachbereich(e)/-gebiet(e): | 18 Fachbereich Elektrotechnik und Informationstechnik 18 Fachbereich Elektrotechnik und Informationstechnik > Integrierte Mikro-Nano-Systeme |
| Hinterlegungsdatum: | 04 Dez 2023 10:21 |
| Letzte Änderung: | 12 Mär 2024 10:18 |
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| Suche nach Titel in: | TUfind oder in Google |
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