Jaeger, Jeannine ; Groher, Florian ; Stamm, Jacqueline ; Spiehl, Dieter ; Braun, Johannes ; Dörsam, Edgar ; Suess, Beatrix (2023)
Characterization and Inkjet Printing of an RNA Aptamer for Paper-Based Biosensing of Ciprofloxacin.
In: Biosensors, 2019, 9 (1)
doi: 10.26083/tuprints-00016878
Artikel, Zweitveröffentlichung, Verlagsversion
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Kurzbeschreibung (Abstract)
The excessive use of antibiotics in food-producing animals causes a steady rise of multiple antibiotic resistance in foodborne bacteria. Next to sulfonamides, the most common antibiotics groups are fluoroquinolones, aminoglycosides, and ß-lactams. Therefore, there is a need for a quick, efficient, and low-cost detection procedure for antibiotics. In this study, we propose an inkjet-printed aptamer-based biosensor developed for the detection of the fluoroquinolone ciprofloxacin. Due to their extraordinary high affinity and specificity, aptamers are already widely used in various applications. Here we present a ciprofloxacin-binding RNA aptamer developed by systematic evolution of ligands by exponential enrichment (SELEX). We characterized the secondary structure of the aptamer and determined the KD to 36 nM that allow detection of antibiotic contamination in a relevant range. We demonstrate that RNA aptamers can be inkjet-printed, dried, and resolved while keeping their functionality consistently intact. With this proof of concept, we are paving the way for a potential range of additional aptamer-based, printable biosensors.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2023 |
| Autor(en): | Jaeger, Jeannine ; Groher, Florian ; Stamm, Jacqueline ; Spiehl, Dieter ; Braun, Johannes ; Dörsam, Edgar ; Suess, Beatrix |
| Art des Eintrags: | Zweitveröffentlichung |
| Titel: | Characterization and Inkjet Printing of an RNA Aptamer for Paper-Based Biosensing of Ciprofloxacin |
| Sprache: | Englisch |
| Publikationsjahr: | 20 November 2023 |
| Ort: | Darmstadt |
| Publikationsdatum der Erstveröffentlichung: | 2019 |
| Ort der Erstveröffentlichung: | Basel |
| Verlag: | MDPI |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Biosensors |
| Jahrgang/Volume einer Zeitschrift: | 9 |
| (Heft-)Nummer: | 1 |
| Kollation: | 10 Seiten |
| DOI: | 10.26083/tuprints-00016878 |
| URL / URN: | https://tuprints.ulb.tu-darmstadt.de/16878 |
| Zugehörige Links: | |
| Herkunft: | Zweitveröffentlichung DeepGreen |
| Kurzbeschreibung (Abstract): | The excessive use of antibiotics in food-producing animals causes a steady rise of multiple antibiotic resistance in foodborne bacteria. Next to sulfonamides, the most common antibiotics groups are fluoroquinolones, aminoglycosides, and ß-lactams. Therefore, there is a need for a quick, efficient, and low-cost detection procedure for antibiotics. In this study, we propose an inkjet-printed aptamer-based biosensor developed for the detection of the fluoroquinolone ciprofloxacin. Due to their extraordinary high affinity and specificity, aptamers are already widely used in various applications. Here we present a ciprofloxacin-binding RNA aptamer developed by systematic evolution of ligands by exponential enrichment (SELEX). We characterized the secondary structure of the aptamer and determined the KD to 36 nM that allow detection of antibiotic contamination in a relevant range. We demonstrate that RNA aptamers can be inkjet-printed, dried, and resolved while keeping their functionality consistently intact. With this proof of concept, we are paving the way for a potential range of additional aptamer-based, printable biosensors. |
| Freie Schlagworte: | biosensor, aptamer, ciprofloxacin, detection, printing, fluorescence |
| Status: | Verlagsversion |
| URN: | urn:nbn:de:tuda-tuprints-168788 |
| Zusätzliche Informationen: | This article belongs to the Special Issue Feature Papers: State-of-the-Art Biosensors Technology 2018 |
| Sachgruppe der Dewey Dezimalklassifikatin (DDC): | 500 Naturwissenschaften und Mathematik > 570 Biowissenschaften, Biologie 600 Technik, Medizin, angewandte Wissenschaften > 620 Ingenieurwissenschaften und Maschinenbau |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie 10 Fachbereich Biologie > Synthetic RNA biology 16 Fachbereich Maschinenbau 16 Fachbereich Maschinenbau > Institut für Druckmaschinen und Druckverfahren (IDD) |
| Hinterlegungsdatum: | 20 Nov 2023 15:07 |
| Letzte Änderung: | 21 Nov 2023 07:31 |
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| Suche nach Titel in: | TUfind oder in Google |
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- Characterization and Inkjet Printing of an RNA Aptamer for Paper-Based Biosensing of Ciprofloxacin. (deposited 20 Nov 2023 15:07) [Gegenwärtig angezeigt]
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