Pastoors, Johannes ; Baltin, Chris ; Bettmer, Jens ; Deitert, Alexander ; Götzen, Tobias ; Michel, Carina ; Deischter, Jeff ; Schroll, Isabel ; Biselli, Andreas ; Palkovits, Regina ; Rose, Marcus ; Jupke, Andreas ; Büchs, Jochen (2023)
Respiration-based investigation of adsorbent-bioprocess compatibility.
In: Biotechnology for Biofuels and Bioproducts, 2023, 16 (1)
doi: 10.26083/tuprints-00024527
Artikel, Zweitveröffentlichung, Verlagsversion
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Kurzbeschreibung (Abstract)
Background: The efficiency of downstream processes plays a crucial role in the transition from conventional petrochemical processes to sustainable biotechnological production routes. One promising candidate for product separation from fermentations with low energy demand and high selectivity is the adsorption of the target product on hydrophobic adsorbents. However, only limited knowledge exists about the interaction of these adsorbents and the bioprocess. The bioprocess could possibly be harmed by the release of inhibitory components from the adsorbent surface. Another possibility is co-adsorption of essential nutrients, especially in an in situ application, making these nutrients unavailable to the applied microorganism.
Results: A test protocol investigating adsorbent-bioprocess compatibility was designed and applied on a variety of adsorbents. Inhibitor release and nutrient adsorption was studied in an isolated manner. Respiratory data recorded by a RAMOS device was used to assess the influence of the adsorbents on the cultivation in three different microbial systems for up to six different adsorbents per system. While no inhibitor release was detected in our investigations, adsorption of different essential nutrients was observed.
Conclusion: The application of adsorption for product recovery from the bioprocess was proven to be generally possible, but nutrient adsorption has to be assessed for each application individually. To account for nutrient adsorption, adsorptive product separation should only be applied after sufficient microbial growth. Moreover, concentrations of co-adsorbed nutrients need to be increased to compensate nutrient loss. The presented protocol enables an investigation of adsorbent-bioprocess compatibility with high-throughput and limited effort.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2023 |
| Autor(en): | Pastoors, Johannes ; Baltin, Chris ; Bettmer, Jens ; Deitert, Alexander ; Götzen, Tobias ; Michel, Carina ; Deischter, Jeff ; Schroll, Isabel ; Biselli, Andreas ; Palkovits, Regina ; Rose, Marcus ; Jupke, Andreas ; Büchs, Jochen |
| Art des Eintrags: | Zweitveröffentlichung |
| Titel: | Respiration-based investigation of adsorbent-bioprocess compatibility |
| Sprache: | Englisch |
| Publikationsjahr: | 20 November 2023 |
| Ort: | Darmstadt |
| Publikationsdatum der Erstveröffentlichung: | 18 März 2023 |
| Ort der Erstveröffentlichung: | London |
| Verlag: | Springer Nature |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Biotechnology for Biofuels and Bioproducts |
| Jahrgang/Volume einer Zeitschrift: | 16 |
| (Heft-)Nummer: | 1 |
| Kollation: | 12 Seiten |
| DOI: | 10.26083/tuprints-00024527 |
| URL / URN: | https://tuprints.ulb.tu-darmstadt.de/24527 |
| Zugehörige Links: | |
| Herkunft: | Zweitveröffentlichungsservice |
| Kurzbeschreibung (Abstract): | Background: The efficiency of downstream processes plays a crucial role in the transition from conventional petrochemical processes to sustainable biotechnological production routes. One promising candidate for product separation from fermentations with low energy demand and high selectivity is the adsorption of the target product on hydrophobic adsorbents. However, only limited knowledge exists about the interaction of these adsorbents and the bioprocess. The bioprocess could possibly be harmed by the release of inhibitory components from the adsorbent surface. Another possibility is co-adsorption of essential nutrients, especially in an in situ application, making these nutrients unavailable to the applied microorganism. Results: A test protocol investigating adsorbent-bioprocess compatibility was designed and applied on a variety of adsorbents. Inhibitor release and nutrient adsorption was studied in an isolated manner. Respiratory data recorded by a RAMOS device was used to assess the influence of the adsorbents on the cultivation in three different microbial systems for up to six different adsorbents per system. While no inhibitor release was detected in our investigations, adsorption of different essential nutrients was observed. Conclusion: The application of adsorption for product recovery from the bioprocess was proven to be generally possible, but nutrient adsorption has to be assessed for each application individually. To account for nutrient adsorption, adsorptive product separation should only be applied after sufficient microbial growth. Moreover, concentrations of co-adsorbed nutrients need to be increased to compensate nutrient loss. The presented protocol enables an investigation of adsorbent-bioprocess compatibility with high-throughput and limited effort. |
| Freie Schlagworte: | Adsorption, RAMOS, Downstream processing, Integrated bioprocesses |
| Status: | Verlagsversion |
| URN: | urn:nbn:de:tuda-tuprints-245275 |
| Sachgruppe der Dewey Dezimalklassifikatin (DDC): | 500 Naturwissenschaften und Mathematik > 540 Chemie |
| Fachbereich(e)/-gebiet(e): | 07 Fachbereich Chemie 07 Fachbereich Chemie > Ernst-Berl-Institut > Fachgebiet Technische Chemie 07 Fachbereich Chemie > Ernst-Berl-Institut > Fachgebiet Technische Chemie > Technische Chemie II |
| Hinterlegungsdatum: | 20 Nov 2023 11:17 |
| Letzte Änderung: | 21 Nov 2023 06:44 |
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| Suche nach Titel in: | TUfind oder in Google |
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