Klein, Thomas ; Funke, Franziska ; Rossbach, Oliver ; Lehmann, Gerhard ; Vockenhuber, Michael ; Medenbach, Jan ; Suess, Beatrix ; Meister, Gunter ; Babinger, Patrick (2023)
Investigating the Prevalence of RNA-Binding Metabolic Enzymes in E. coli.
In: International Journal of Molecular Sciences, 24 (14)
doi: 10.3390/ijms241411536
Artikel, Bibliographie

Kurzbeschreibung (Abstract)

An open research field in cellular regulation is the assumed crosstalk between RNAs, metabolic enzymes, and metabolites, also known as the REM hypothesis. High-throughput assays have produced extensive interactome data with metabolic enzymes frequently found as hits, but only a few examples have been biochemically validated, with deficits especially in prokaryotes. Therefore, we rationally selected nineteen enzymes from such datasets and examined their ability to bind RNAs using two complementary methods, iCLIP and SELEX. Found interactions were validated by EMSA and other methods. For most of the candidates, we observed no RNA binding (12/19) or a rather unspecific binding (5/19). Two of the candidates, namely glutamate-5-kinase (ProB) and quinone oxidoreductase (QorA), displayed specific and previously unknown binding to distinct RNAs. We concentrated on the interaction of QorA to the mRNA of , a grounded prophage gene, which could be validated by EMSA and MST. Because the physiological function of both partners is not known, the biological relevance of this interaction remains elusive. Furthermore, we found novel RNA targets for the MS2 phage coat protein that served us as control. Our results indicate that RNA binding of metabolic enzymes in procaryotes is less frequent than suggested by the results of high-throughput studies, but does occur.

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