Knecht, Stephan ; Blanchard, John W. ; Barskiy, Danila ; Cavallari, Eleonora ; Dagys, Laurynas ; Dyke, Erik Van ; Tsukanov, Maksim ; Bliemel, Bea ; Münnemann, Kerstin ; Aime, Silvio ; Reineri, Francesca ; Levitt, Malcolm H. ; Buntkowsky, Gerd ; Pines, Alexander ; Blümler, Peter ; Budker, Dmitry ; Eills, James (2023)
Rapid hyperpolarization and purification of the metabolite fumarate in aqueous solution.
In: Proceedings of the National Academy of Sciences of the United States of America : PNAS, 2021, 118 (13)
doi: 10.26083/tuprints-00024221
Artikel, Zweitveröffentlichung, Verlagsversion

Kurzbeschreibung (Abstract)

Hyperpolarized fumarate is a promising biosensor for carbon-13 magnetic resonance metabolic imaging. Such molecular imaging applications require nuclear hyperpolarization to attain sufficient signal strength. Dissolution dynamic nuclear polarization is the current state-of-the-art methodology for hyperpolarizing fumarate, but this is expensive and relatively slow. Alternatively, this important biomolecule can be hyperpolarized in a cheap and convenient manner using parahydrogen-induced polarization. However, this process requires a chemical reaction, and the resulting solutions are contaminated with the catalyst, unreacted reagents, and reaction side-product molecules, and are hence unsuitable for use in vivo. In this work we show that the hyperpolarized fumarate can be purified from these contaminants by acid precipitation as a pure solid, and later redissolved to a desired concentration in a clean aqueous solvent. Significant advances in the reaction conditions and reactor equipment allow for formation of hyperpolarized fumarate at ¹³C polarization levels of 30–45%.

Frage zum Eintrag

Redaktionelle Details anzeigen