Puskar, Ljiljana ; Tuckermann, Rudolf ; Frosch, Torsten ; Popp, Jürgen ; Ly, Vanalysa ; McNaughton, Don ; Wood, Bayden R. (2007)
Raman acoustic levitation spectroscopy of red blood cells and Plasmodium falciparum trophozoites.
In: Lab on a Chip, 7 (9)
doi: 10.1039/b706997a
Artikel, Bibliographie
Kurzbeschreibung (Abstract)
Methods to probe the molecular structure of living cells are of paramount importance in understanding drug interactions and environmental influences in these complex dynamical systems. The coupling of an acoustic levitation device with a micro-Raman spectrometer provides a direct molecular probe of cellular chemistry in a containerless environment minimizing signal attenuation and eliminating the affects of adhesion to walls and interfaces. We show that the Raman acoustic levitation spectroscopic (RALS) approach can be used to monitor the heme dynamics of a levitated 5 µL suspension of red blood cells and to detect hemozoin in malaria infected cells. The spectra obtained have an excellent signal-to-noise ratio and demonstrate for the first time the utility of the technique as a diagnostic and monitoring tool for minute sample volumes of living animal cells.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2007 |
| Autor(en): | Puskar, Ljiljana ; Tuckermann, Rudolf ; Frosch, Torsten ; Popp, Jürgen ; Ly, Vanalysa ; McNaughton, Don ; Wood, Bayden R. |
| Art des Eintrags: | Bibliographie |
| Titel: | Raman acoustic levitation spectroscopy of red blood cells and Plasmodium falciparum trophozoites |
| Sprache: | Englisch |
| Publikationsjahr: | 3 August 2007 |
| Verlag: | Royal Society of Chemistry |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Lab on a Chip |
| Jahrgang/Volume einer Zeitschrift: | 7 |
| (Heft-)Nummer: | 9 |
| DOI: | 10.1039/b706997a |
| Kurzbeschreibung (Abstract): | Methods to probe the molecular structure of living cells are of paramount importance in understanding drug interactions and environmental influences in these complex dynamical systems. The coupling of an acoustic levitation device with a micro-Raman spectrometer provides a direct molecular probe of cellular chemistry in a containerless environment minimizing signal attenuation and eliminating the affects of adhesion to walls and interfaces. We show that the Raman acoustic levitation spectroscopic (RALS) approach can be used to monitor the heme dynamics of a levitated 5 µL suspension of red blood cells and to detect hemozoin in malaria infected cells. The spectra obtained have an excellent signal-to-noise ratio and demonstrate for the first time the utility of the technique as a diagnostic and monitoring tool for minute sample volumes of living animal cells. |
| Freie Schlagworte: | Biomedical Sensing, Red Blood Cells, Malaria, Hemozoin, Raman Acoustic Levitation Spectroscopy, Biomedical Pharmaceutical Spectroscopy, Resonance Raman Spectroscopy |
| Fachbereich(e)/-gebiet(e): | 18 Fachbereich Elektrotechnik und Informationstechnik 18 Fachbereich Elektrotechnik und Informationstechnik > Biophotonik-Medizintechnik |
| Hinterlegungsdatum: | 19 Jan 2024 10:39 |
| Letzte Änderung: | 19 Jan 2024 18:02 |
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