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Raman spectroscopic investigation of 13CO2 labeling and leaf dark respiration of Fagus sylvatica L. (European beech)

Keiner, Robert ; Gruselle, Marie-Cécile ; Michalzik, Beate ; Popp, Jürgen ; Frosch, Torsten (2015)
Raman spectroscopic investigation of 13CO2 labeling and leaf dark respiration of Fagus sylvatica L. (European beech).
In: Analytical and Bioanalytical Chemistry, 407 (7)
doi: 10.1007/s00216-014-8446-8
Artikel, Bibliographie

Kurzbeschreibung (Abstract)

An important issue, in times of climate change and more extreme weather events, is the investigation of forest ecosystem reactions to these events. Longer drought periods stress the vitality of trees and promote mass insect outbreaks, which strongly affect ecosystem processes and services. Cavity-enhanced Raman gas spectrometry was applied for online multi-gas analysis of the gas exchange rates of O2 and CO2 and the labeling of Fagus sylvatica L. (European beech) seedlings with 13CO2. The rapid monitoring of all these gases simultaneously allowed for the separation of photosynthetic uptake of CO2 by the beech seedlings and a constant 12CO2 efflux via respiration and thus for a correction of the measured 12CO2 concentrations in course of the labeling experiment. The effects of aphid infestation with the woolly beech aphid (Phyllaphis fagi L.) as well as the effect of a drought period on the respirational gas exchange were investigated. A slightly decreased respirational activity of drought-stressed seedlings in comparison to normally watered seedlings was found already for a low drought intensity. Cavity-enhanced Raman gas monitoring of O2, 12CO2, and 13CO2 was proven to be a powerful new tool for studying the effect of drought stress and aphid infestation on the respirational activity of European beech seedlings as an example of important forest species in Central Europe.

Typ des Eintrags: Artikel
Erschienen: 2015
Autor(en): Keiner, Robert ; Gruselle, Marie-Cécile ; Michalzik, Beate ; Popp, Jürgen ; Frosch, Torsten
Art des Eintrags: Bibliographie
Titel: Raman spectroscopic investigation of 13CO2 labeling and leaf dark respiration of Fagus sylvatica L. (European beech)
Sprache: Englisch
Publikationsjahr: 11 Januar 2015
Verlag: Springer
Titel der Zeitschrift, Zeitung oder Schriftenreihe: Analytical and Bioanalytical Chemistry
Jahrgang/Volume einer Zeitschrift: 407
(Heft-)Nummer: 7
DOI: 10.1007/s00216-014-8446-8
Kurzbeschreibung (Abstract):

An important issue, in times of climate change and more extreme weather events, is the investigation of forest ecosystem reactions to these events. Longer drought periods stress the vitality of trees and promote mass insect outbreaks, which strongly affect ecosystem processes and services. Cavity-enhanced Raman gas spectrometry was applied for online multi-gas analysis of the gas exchange rates of O2 and CO2 and the labeling of Fagus sylvatica L. (European beech) seedlings with 13CO2. The rapid monitoring of all these gases simultaneously allowed for the separation of photosynthetic uptake of CO2 by the beech seedlings and a constant 12CO2 efflux via respiration and thus for a correction of the measured 12CO2 concentrations in course of the labeling experiment. The effects of aphid infestation with the woolly beech aphid (Phyllaphis fagi L.) as well as the effect of a drought period on the respirational gas exchange were investigated. A slightly decreased respirational activity of drought-stressed seedlings in comparison to normally watered seedlings was found already for a low drought intensity. Cavity-enhanced Raman gas monitoring of O2, 12CO2, and 13CO2 was proven to be a powerful new tool for studying the effect of drought stress and aphid infestation on the respirational activity of European beech seedlings as an example of important forest species in Central Europe.

Freie Schlagworte: 13C Isotope Labeling, isotopic labeling, stable isotope probing, Leaf Dark Respiration, Respiration Quotient RQ, Plant, Beech, Cavity Enhanced Raman Spectroscopy CERS, Environmental Gas Sensing, Ecological Gas Sensing, Gas Analysis
Fachbereich(e)/-gebiet(e): 18 Fachbereich Elektrotechnik und Informationstechnik
18 Fachbereich Elektrotechnik und Informationstechnik > Biophotonik-Medizintechnik
Hinterlegungsdatum: 19 Jan 2024 10:20
Letzte Änderung: 30 Apr 2024 10:48
PPN: 517674955
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