Knebl, Andreas ; Domes, Robert ; Wolf, Sebastian ; Domes, Christian ; Popp, Juergen ; Frosch, Torsten (2020)
Fiber-Enhanced Raman Gas Spectroscopy for the Study of Microbial Methanogenesis.
In: Analytical Chemistry, 92 (18)
doi: 10.1021/acs.analchem.0c02507
Artikel, Bibliographie
Kurzbeschreibung (Abstract)
Microbial methanogenesis is a key biogeochemical process in the carbon cycle that is responsible for 70% of global emissions of the potent greenhouse gas methane (CH4). Further knowledge about microbial methanogenesis is crucial to mitigate emissions, increase climate model accuracy, or advance methanogenic biogas production. The current understanding of the substrate use of methanogenic microbes is limited, especially regarding the methylotrophic pathway. Here, we present fiber-enhanced Raman spectroscopy (FERS) of headspace gases as an alternate tool to study methanogenesis and substrate use in particular. The optical technique is nondestructive and sensitive to CH4, hydrogen (H2), and carbon dioxide with a large dynamic range from trace levels (demonstrated LoDs: CH4, 3 ppm; H2, 49 ppm) to pure gases. In addition, the portable FERS system can provide quantitative information about methanol concentration in the liquid phase of microbial cultures through headspace gas sampling (LoD 25 ppm). We demonstrate how FERS gas sensing could enable us to track substrate and product levels of microbial methanogenesis with just one instrument. The versatility of Raman gas spectroscopy could moreover help us to elucidate links between nitrogen and carbon cycle in microbial communities in the near future.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2020 |
| Autor(en): | Knebl, Andreas ; Domes, Robert ; Wolf, Sebastian ; Domes, Christian ; Popp, Juergen ; Frosch, Torsten |
| Art des Eintrags: | Bibliographie |
| Titel: | Fiber-Enhanced Raman Gas Spectroscopy for the Study of Microbial Methanogenesis |
| Sprache: | Englisch |
| Publikationsjahr: | 26 August 2020 |
| Verlag: | ACS Publications |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Analytical Chemistry |
| Jahrgang/Volume einer Zeitschrift: | 92 |
| (Heft-)Nummer: | 18 |
| DOI: | 10.1021/acs.analchem.0c02507 |
| Kurzbeschreibung (Abstract): | Microbial methanogenesis is a key biogeochemical process in the carbon cycle that is responsible for 70% of global emissions of the potent greenhouse gas methane (CH4). Further knowledge about microbial methanogenesis is crucial to mitigate emissions, increase climate model accuracy, or advance methanogenic biogas production. The current understanding of the substrate use of methanogenic microbes is limited, especially regarding the methylotrophic pathway. Here, we present fiber-enhanced Raman spectroscopy (FERS) of headspace gases as an alternate tool to study methanogenesis and substrate use in particular. The optical technique is nondestructive and sensitive to CH4, hydrogen (H2), and carbon dioxide with a large dynamic range from trace levels (demonstrated LoDs: CH4, 3 ppm; H2, 49 ppm) to pure gases. In addition, the portable FERS system can provide quantitative information about methanol concentration in the liquid phase of microbial cultures through headspace gas sampling (LoD 25 ppm). We demonstrate how FERS gas sensing could enable us to track substrate and product levels of microbial methanogenesis with just one instrument. The versatility of Raman gas spectroscopy could moreover help us to elucidate links between nitrogen and carbon cycle in microbial communities in the near future. |
| Freie Schlagworte: | Fiber Enhanced Raman Spectroscopy FERS, Microbial Methanogenesis, Green Energy, Environmental Gas Sensing, Gas Analysis, Bacteria, Volatile Organic Compounds VOCs, hollow core antiresonant fiber |
| Fachbereich(e)/-gebiet(e): | 18 Fachbereich Elektrotechnik und Informationstechnik 18 Fachbereich Elektrotechnik und Informationstechnik > Biophotonik-Medizintechnik |
| Hinterlegungsdatum: | 18 Jan 2024 10:33 |
| Letzte Änderung: | 19 Mär 2024 09:03 |
| PPN: | 515994650 |
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