Wolf, Sebastian ; Popp, Jürgen ; Frosch, Torsten (2023)
Multifocal hyperspectral Raman imaging setup for multi-well plates.
In: Sensors and Actuators B: Chemical, 375
doi: 10.1016/j.snb.2022.132949
Artikel, Bibliographie
Kurzbeschreibung (Abstract)
High-throughput screening has become widespread in drug discovery. This increases the need for efficient, parallelized spectral imaging techniques. However, current designs focus on microplate readers without the capability of measuring hyperspectral images or microscopy setups. In this work, a novel setup for parallelized hyperspectral Raman spectroscopy is presented. Utilizing a unique combination of a diffractive element and a micro-lens array in a tailored optical setup, it was possible to acquire 64 high-resolution Raman spectra from a multi-well plate simultaneously. This allows an enormous reduction in measurement time in comparison to sequential readers. Thus, the presented parallelized hyperspectral Raman spectroscopic setup provides a time saving way for modern high-throughput screening applications and the advantage of chemically selective real-time comparison between the different micro-well spots.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2023 |
| Autor(en): | Wolf, Sebastian ; Popp, Jürgen ; Frosch, Torsten |
| Art des Eintrags: | Bibliographie |
| Titel: | Multifocal hyperspectral Raman imaging setup for multi-well plates |
| Sprache: | Englisch |
| Publikationsjahr: | 15 Januar 2023 |
| Verlag: | Elsevier |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Sensors and Actuators B: Chemical |
| Jahrgang/Volume einer Zeitschrift: | 375 |
| DOI: | 10.1016/j.snb.2022.132949 |
| Kurzbeschreibung (Abstract): | High-throughput screening has become widespread in drug discovery. This increases the need for efficient, parallelized spectral imaging techniques. However, current designs focus on microplate readers without the capability of measuring hyperspectral images or microscopy setups. In this work, a novel setup for parallelized hyperspectral Raman spectroscopy is presented. Utilizing a unique combination of a diffractive element and a micro-lens array in a tailored optical setup, it was possible to acquire 64 high-resolution Raman spectra from a multi-well plate simultaneously. This allows an enormous reduction in measurement time in comparison to sequential readers. Thus, the presented parallelized hyperspectral Raman spectroscopic setup provides a time saving way for modern high-throughput screening applications and the advantage of chemically selective real-time comparison between the different micro-well spots. |
| Freie Schlagworte: | multifocal imaging, high throughput Chemical Imaging, Raman Difference Spectroscopy RDS, hyperspectral, molecular interaction, Biomedical Spectroscopy, Pharmaceutical Spectroscopy |
| Zusätzliche Informationen: | Art.No.: 132949 |
| Fachbereich(e)/-gebiet(e): | 18 Fachbereich Elektrotechnik und Informationstechnik 18 Fachbereich Elektrotechnik und Informationstechnik > Biophotonik-Medizintechnik |
| Hinterlegungsdatum: | 18 Jan 2024 09:48 |
| Letzte Änderung: | 26 Feb 2024 07:10 |
| PPN: | 515740101 |
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