Zhang, Wenbo ; Bertinetti, Luca ; Yavuzsoy, Efe Cuma ; Gao, Changyou ; Schneck, Emanuel ; Fratzl, Peter (2022)
Submicron-sized in-situ osmotic pressure sensors for in-vitro applications in biology.
doi: 10.1101/2022.09.13.507780
Report, Bibliographie
Kurzbeschreibung (Abstract)
Physical forces are important cues in determining the development and the normal function of biological tissues. While forces generated by molecular motors have been widely studied, forces resulting from osmotic gradients have been less considered in this context. A possible reason is the lack of direct in-situ measurement methods that can be applied to cell and organ culture systems. Herein, novel kinds of FRET (resonance energy transfer)-based liposomal sensors are developed, so that their sensing range and sensitivity can be adjusted to satisfy physiological osmotic conditions. Several types of sensors are prepared, either based on PEGylated liposomes with steric stabilization and stealth property or on crosslinked liposomes capable of enduring relatively harsh environments for liposomes (e.g. in the presence of biosurfactants). The sensors are demonstrated to be effective in the measurement of osmotic pressures in pre-osteoblastic in-vitro cell culture systems by means of FRET microscopy. This development paves the way towards the in-situ sensing of osmotic pressures in biological culture systems.
| Typ des Eintrags: | Report |
|---|---|
| Erschienen: | 2022 |
| Autor(en): | Zhang, Wenbo ; Bertinetti, Luca ; Yavuzsoy, Efe Cuma ; Gao, Changyou ; Schneck, Emanuel ; Fratzl, Peter |
| Art des Eintrags: | Bibliographie |
| Titel: | Submicron-sized in-situ osmotic pressure sensors for in-vitro applications in biology |
| Sprache: | Englisch |
| Publikationsjahr: | 15 September 2022 |
| Verlag: | bioRxiv |
| Reihe: | Biophysics |
| DOI: | 10.1101/2022.09.13.507780 |
| Zugehörige Links: | |
| Kurzbeschreibung (Abstract): | Physical forces are important cues in determining the development and the normal function of biological tissues. While forces generated by molecular motors have been widely studied, forces resulting from osmotic gradients have been less considered in this context. A possible reason is the lack of direct in-situ measurement methods that can be applied to cell and organ culture systems. Herein, novel kinds of FRET (resonance energy transfer)-based liposomal sensors are developed, so that their sensing range and sensitivity can be adjusted to satisfy physiological osmotic conditions. Several types of sensors are prepared, either based on PEGylated liposomes with steric stabilization and stealth property or on crosslinked liposomes capable of enduring relatively harsh environments for liposomes (e.g. in the presence of biosurfactants). The sensors are demonstrated to be effective in the measurement of osmotic pressures in pre-osteoblastic in-vitro cell culture systems by means of FRET microscopy. This development paves the way towards the in-situ sensing of osmotic pressures in biological culture systems. |
| Zusätzliche Informationen: | Später erschienen in Advanced Healthcare Materials 2023,12, 2202373 |
| Fachbereich(e)/-gebiet(e): | 05 Fachbereich Physik 05 Fachbereich Physik > Institut für Physik Kondensierter Materie (IPKM) 05 Fachbereich Physik > Institut für Physik Kondensierter Materie (IPKM) > Physik biologischer weicher Materie |
| Hinterlegungsdatum: | 09 Dez 2022 09:05 |
| Letzte Änderung: | 10 Aug 2023 13:26 |
| PPN: | 507616332 |
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