Kraus, Leon ; Suess, Beatrix (2023)
RNA Capture-SELEX on Streptavidin Magnetic Beads.
In: Nucleic Acid Aptamers: Selection, Characterization, and Application, Auflage: 2nd Edition
doi: 10.1007/978-1-0716-2695-5_5
Buchkapitel, Bibliographie
Kurzbeschreibung (Abstract)
SELEX has enabled the selection of aptamers, nucleic acids that can bind a defined ligand, in some cases with exceptionally high affinity and specificity. The SELEX protocol has been adapted many times to fit a variety of needs. This protocol describes such an adaptation, namely, RNA-Capture SELEX that we have used to successfully develop small molecule-binding RNA aptamers. Our proposed method specifically selects not only for excellent binding but also for conformational switching. In consequence, we found this SELEX method to be particularly suitable for identifying aptamers for further application in synthetic riboswitch engineering.
| Typ des Eintrags: | Buchkapitel |
|---|---|
| Erschienen: | 2023 |
| Autor(en): | Kraus, Leon ; Suess, Beatrix |
| Art des Eintrags: | Bibliographie |
| Titel: | RNA Capture-SELEX on Streptavidin Magnetic Beads |
| Sprache: | Englisch |
| Publikationsjahr: | 2023 |
| Verlag: | Humana Press |
| Buchtitel: | Nucleic Acid Aptamers: Selection, Characterization, and Application |
| Reihe: | Methods in molecular biology |
| Band einer Reihe: | 2570 |
| Auflage: | 2nd Edition |
| DOI: | 10.1007/978-1-0716-2695-5_5 |
| Kurzbeschreibung (Abstract): | SELEX has enabled the selection of aptamers, nucleic acids that can bind a defined ligand, in some cases with exceptionally high affinity and specificity. The SELEX protocol has been adapted many times to fit a variety of needs. This protocol describes such an adaptation, namely, RNA-Capture SELEX that we have used to successfully develop small molecule-binding RNA aptamers. Our proposed method specifically selects not only for excellent binding but also for conformational switching. In consequence, we found this SELEX method to be particularly suitable for identifying aptamers for further application in synthetic riboswitch engineering. |
| ID-Nummer: | pmid:36156774 |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie 10 Fachbereich Biologie > Synthetic RNA biology |
| Hinterlegungsdatum: | 10 Okt 2022 10:22 |
| Letzte Änderung: | 25 Jul 2023 08:24 |
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