Pusterla, Julio ; Scoppola, Ernesto ; Appel, Christian ; Mukhina, Tetiana ; Shen, Chen ; Brezesinski, Gerald ; Schneck, Emanuel (2022)
Characterization of lipid bilayers adsorbed to functionalized air/water interfaces.
In: Nanoscale, (Early Access)
doi: 10.1039/D2NR03334H
Artikel, Bibliographie
Kurzbeschreibung (Abstract)
Lipid bilayers immobilized in planar geometries, such as solid-supported or “floating” bilayers, have enabled detailed studies of biological membranes with numerous experimental techniques, notably X-ray and neutron reflectometry. However, the presence of a solid support also has disadvantages as it complicates the use of spectroscopic techniques as well as surface rheological measurements that would require surface deformations. Here, in order to overcome these limitations, we investigate lipid bilayers adsorbed to inherently soft and experimentally well accessible air/water interfaces that are functionalized with Langmuir monolayers of amphiphiles. The bilayers are characterized with ellipsometry, X-ray scattering, and X-ray fluorescence. Grazing-incidence X-ray diffraction reveals that lipid bilayers in a chain-ordered state can have significantly different structural features than regular Langmuir monolayers of the same composition. Our results suggest that bilayers at air/water interfaces may be well suited for fundamental studies in the field of membrane biophysics.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2022 |
| Autor(en): | Pusterla, Julio ; Scoppola, Ernesto ; Appel, Christian ; Mukhina, Tetiana ; Shen, Chen ; Brezesinski, Gerald ; Schneck, Emanuel |
| Art des Eintrags: | Bibliographie |
| Titel: | Characterization of lipid bilayers adsorbed to functionalized air/water interfaces |
| Sprache: | Englisch |
| Publikationsjahr: | 3 Oktober 2022 |
| Verlag: | Royal Society of Chemistry |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Nanoscale |
| (Heft-)Nummer: | Early Access |
| DOI: | 10.1039/D2NR03334H |
| Kurzbeschreibung (Abstract): | Lipid bilayers immobilized in planar geometries, such as solid-supported or “floating” bilayers, have enabled detailed studies of biological membranes with numerous experimental techniques, notably X-ray and neutron reflectometry. However, the presence of a solid support also has disadvantages as it complicates the use of spectroscopic techniques as well as surface rheological measurements that would require surface deformations. Here, in order to overcome these limitations, we investigate lipid bilayers adsorbed to inherently soft and experimentally well accessible air/water interfaces that are functionalized with Langmuir monolayers of amphiphiles. The bilayers are characterized with ellipsometry, X-ray scattering, and X-ray fluorescence. Grazing-incidence X-ray diffraction reveals that lipid bilayers in a chain-ordered state can have significantly different structural features than regular Langmuir monolayers of the same composition. Our results suggest that bilayers at air/water interfaces may be well suited for fundamental studies in the field of membrane biophysics. |
| Fachbereich(e)/-gebiet(e): | 05 Fachbereich Physik 05 Fachbereich Physik > Institut für Physik Kondensierter Materie (IPKM) 05 Fachbereich Physik > Institut für Physik Kondensierter Materie (IPKM) > Physik biologischer weicher Materie 05 Fachbereich Physik > Institut für Physik Kondensierter Materie (IPKM) > Theorie weicher Materie |
| Hinterlegungsdatum: | 10 Okt 2022 12:56 |
| Letzte Änderung: | 02 Mär 2023 13:08 |
| PPN: | 505412659 |
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