Kübelbeck, Sonja ; Mikhael, Jules ; Keller, Harald ; Konradi, Rupert ; Andrieu-Brunsen, Annette ; Baier, Grit (2022)
Enzyme-Polymer Conjugates to Enhance Enzyme Shelf Life in a Liquid Detergent Formulation.
In: Macromolecular Bioscience, 2018, 18 (7)
doi: 10.26083/tuprints-00021680
Artikel, Zweitveröffentlichung, Postprint
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Kurzbeschreibung (Abstract)
Herein, the synthesis of enzyme–polymer conjugates is reported. Four different activated polymers (mPEG-aldehyde, mPEG-NHS, maltodextrin-aldehyde, carboxymethyl cellulose aldehyde) are conjugated to the surface of protease, α-amylase, and lipase using two different strategies (reductive amination and alkylation with NHS-activated acid). Although the chemical modification of the enzymes is accompanied by losses in enzyme activity (maximum loss 40%), the covalent attachment of polymers increases the thermal stability and the stability in a standard detergent formulation compared to the unmodified enzymes. The enzyme–polymer conjugates are characterized by asymmetrical-flow field-flow fractionation and differential scanning microcalorimetry. Furthermore, it is demonstrated that conjugated enzymes still show performance in a real washing process. Enzyme–polymer conjugates show a potential as a stabilizing system for enzymes in detergents.
Typ des Eintrags: | Artikel |
---|---|
Erschienen: | 2022 |
Autor(en): | Kübelbeck, Sonja ; Mikhael, Jules ; Keller, Harald ; Konradi, Rupert ; Andrieu-Brunsen, Annette ; Baier, Grit |
Art des Eintrags: | Zweitveröffentlichung |
Titel: | Enzyme-Polymer Conjugates to Enhance Enzyme Shelf Life in a Liquid Detergent Formulation |
Sprache: | Englisch |
Publikationsjahr: | 2022 |
Ort: | Darmstadt |
Publikationsdatum der Erstveröffentlichung: | 2018 |
Verlag: | John Wiley & Sons |
Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Macromolecular Bioscience |
Jahrgang/Volume einer Zeitschrift: | 18 |
(Heft-)Nummer: | 7 |
Kollation: | 26 Seiten |
DOI: | 10.26083/tuprints-00021680 |
URL / URN: | https://tuprints.ulb.tu-darmstadt.de/21680 |
Zugehörige Links: | |
Herkunft: | Zweitveröffentlichungsservice |
Kurzbeschreibung (Abstract): | Herein, the synthesis of enzyme–polymer conjugates is reported. Four different activated polymers (mPEG-aldehyde, mPEG-NHS, maltodextrin-aldehyde, carboxymethyl cellulose aldehyde) are conjugated to the surface of protease, α-amylase, and lipase using two different strategies (reductive amination and alkylation with NHS-activated acid). Although the chemical modification of the enzymes is accompanied by losses in enzyme activity (maximum loss 40%), the covalent attachment of polymers increases the thermal stability and the stability in a standard detergent formulation compared to the unmodified enzymes. The enzyme–polymer conjugates are characterized by asymmetrical-flow field-flow fractionation and differential scanning microcalorimetry. Furthermore, it is demonstrated that conjugated enzymes still show performance in a real washing process. Enzyme–polymer conjugates show a potential as a stabilizing system for enzymes in detergents. |
Status: | Postprint |
URN: | urn:nbn:de:tuda-tuprints-216808 |
Sachgruppe der Dewey Dezimalklassifikatin (DDC): | 500 Naturwissenschaften und Mathematik > 540 Chemie |
Fachbereich(e)/-gebiet(e): | Profilbereiche Profilbereiche > Thermo-Fluids & Interfaces 07 Fachbereich Chemie 07 Fachbereich Chemie > Ernst-Berl-Institut 07 Fachbereich Chemie > Ernst-Berl-Institut > Fachgebiet Makromolekulare Chemie |
Hinterlegungsdatum: | 20 Jul 2022 12:17 |
Letzte Änderung: | 03 Jul 2024 02:30 |
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- Enzyme-Polymer Conjugates to Enhance Enzyme Shelf Life in a Liquid Detergent Formulation. (deposited 20 Jul 2022 12:17) [Gegenwärtig angezeigt]
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