Fischer, Ulrike ; Rheinheimer, Stefanie ; Krempler, Andrea ; Löbrich, Markus ; Meese, Eckart (2021)
Glioma-amplified sequence KUB3 influences double-strand break repair after ionizing radiation.
In: International Journal of Oncology, 2013, 43 (1)
doi: 10.26083/tuprints-00019034
Artikel, Zweitveröffentlichung, Verlagsversion
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Kurzbeschreibung (Abstract)
Human glioblastomas are characterized by frequent DNA amplifications most often at chromosome regions 7p11.2 and 12q13-15. Although amplification is a well-known hallmark of glioblastoma genetics the function of most amplified genes in glioblastoma biology is not understood. Previously, we cloned Ku70-binding protein 3 (KUB3) from the amplified domain at 12q13-15. Here, we report that glioblastoma cell cultures with endogenous KUB3 gene amplification and with elevated KUB3 protein expression show an efficient double-strand break (DSB) repair after being irradiated with 1 Gy. A significantly less efficient DSB repair was found in glioma cell cultures without KUB3 amplification and expression. Furthermore, we found that a siRNA-mediated reduction of the endogenous KUB3 expression in glioblastoma cells resulted in a reduction of the repair efficiency. HeLa cells transfected with KUB3 showed an increased DSB repair in comparison to untreated HeLa cells. In addition, KUB3 seems to influence DSB efficiency via the DNA-PK-dependent repair pathway as shown by simultaneous inhibition of KUB3 and DNA-PK. The data provide the first evidence for a link between the level of KUB3 amplification and expression in glioma and DSB repair efficiency.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2021 |
| Autor(en): | Fischer, Ulrike ; Rheinheimer, Stefanie ; Krempler, Andrea ; Löbrich, Markus ; Meese, Eckart |
| Art des Eintrags: | Zweitveröffentlichung |
| Titel: | Glioma-amplified sequence KUB3 influences double-strand break repair after ionizing radiation |
| Sprache: | Englisch |
| Publikationsjahr: | 2021 |
| Publikationsdatum der Erstveröffentlichung: | 2013 |
| Verlag: | Spandidos Publications |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | International Journal of Oncology |
| Jahrgang/Volume einer Zeitschrift: | 43 |
| (Heft-)Nummer: | 1 |
| DOI: | 10.26083/tuprints-00019034 |
| URL / URN: | https://tuprints.ulb.tu-darmstadt.de/19034 |
| Zugehörige Links: | |
| Herkunft: | Zweitveröffentlichungsservice |
| Kurzbeschreibung (Abstract): | Human glioblastomas are characterized by frequent DNA amplifications most often at chromosome regions 7p11.2 and 12q13-15. Although amplification is a well-known hallmark of glioblastoma genetics the function of most amplified genes in glioblastoma biology is not understood. Previously, we cloned Ku70-binding protein 3 (KUB3) from the amplified domain at 12q13-15. Here, we report that glioblastoma cell cultures with endogenous KUB3 gene amplification and with elevated KUB3 protein expression show an efficient double-strand break (DSB) repair after being irradiated with 1 Gy. A significantly less efficient DSB repair was found in glioma cell cultures without KUB3 amplification and expression. Furthermore, we found that a siRNA-mediated reduction of the endogenous KUB3 expression in glioblastoma cells resulted in a reduction of the repair efficiency. HeLa cells transfected with KUB3 showed an increased DSB repair in comparison to untreated HeLa cells. In addition, KUB3 seems to influence DSB efficiency via the DNA-PK-dependent repair pathway as shown by simultaneous inhibition of KUB3 and DNA-PK. The data provide the first evidence for a link between the level of KUB3 amplification and expression in glioma and DSB repair efficiency. |
| Status: | Verlagsversion |
| URN: | urn:nbn:de:tuda-tuprints-190343 |
| Sachgruppe der Dewey Dezimalklassifikatin (DDC): | 500 Naturwissenschaften und Mathematik > 570 Biowissenschaften, Biologie |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie 10 Fachbereich Biologie > Radiation Biology and DNA Repair |
| Hinterlegungsdatum: | 07 Sep 2021 12:12 |
| Letzte Änderung: | 13 Sep 2021 06:31 |
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| Suche nach Titel in: | TUfind oder in Google |
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- Glioma-amplified sequence KUB3 influences double-strand break repair after ionizing radiation. (deposited 07 Sep 2021 12:12) [Gegenwärtig angezeigt]
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