Peter, Stephen A. ; Isaac, Jessica S. ; Narberhaus, Franz ; Weigand, Julia E. (2021)
A novel, universally active C-terminal protein degradation signal generated by alternative splicing.
In: Journal of molecular biology, 433 (8)
doi: 10.1016/j.jmb.2021.166890
Artikel, Bibliographie
Kurzbeschreibung (Abstract)
Proteome integrity is crucial for cellular homeostasis and adaptation to stress conditions such as hypoxia. One mechanism for rapid adaptation of the proteome in response to changing environmental signals is alternative splicing. In addition to generating different protein isoforms, alternative splicing is also capable of controlling total protein levels by the regulated synthesis of non-productive mRNA isoforms. The hypoxia-induced isoform E of the tumor suppressor MAX is produced by retention and translation of the last intron. This leads to an alternative C-terminus that harbors a potent C-degron, the isoE degron. Strikingly, the isoE degron represents a universal protein degradation signal that is not only functional in mammalian cells, but also in yeast and even in bacteria. Essential for efficient protein decay is a conserved (F/W)xxW motif. Degradation of isoE tagged proteins is mediated by the proteasome in eukaryotes and Lon protease in bacteria. Thus, the isoE degron is a broadly applicable and highly efficient tool in protein analyses.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2021 |
| Autor(en): | Peter, Stephen A. ; Isaac, Jessica S. ; Narberhaus, Franz ; Weigand, Julia E. |
| Art des Eintrags: | Bibliographie |
| Titel: | A novel, universally active C-terminal protein degradation signal generated by alternative splicing |
| Sprache: | Englisch |
| Publikationsjahr: | 23 Februar 2021 |
| Verlag: | Elsevier |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Journal of molecular biology |
| Jahrgang/Volume einer Zeitschrift: | 433 |
| (Heft-)Nummer: | 8 |
| DOI: | 10.1016/j.jmb.2021.166890 |
| Kurzbeschreibung (Abstract): | Proteome integrity is crucial for cellular homeostasis and adaptation to stress conditions such as hypoxia. One mechanism for rapid adaptation of the proteome in response to changing environmental signals is alternative splicing. In addition to generating different protein isoforms, alternative splicing is also capable of controlling total protein levels by the regulated synthesis of non-productive mRNA isoforms. The hypoxia-induced isoform E of the tumor suppressor MAX is produced by retention and translation of the last intron. This leads to an alternative C-terminus that harbors a potent C-degron, the isoE degron. Strikingly, the isoE degron represents a universal protein degradation signal that is not only functional in mammalian cells, but also in yeast and even in bacteria. Essential for efficient protein decay is a conserved (F/W)xxW motif. Degradation of isoE tagged proteins is mediated by the proteasome in eukaryotes and Lon protease in bacteria. Thus, the isoE degron is a broadly applicable and highly efficient tool in protein analyses. |
| ID-Nummer: | pmid:33636164 |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie 10 Fachbereich Biologie > RNA Biochemie |
| Hinterlegungsdatum: | 05 Mär 2021 08:49 |
| Letzte Änderung: | 05 Mär 2021 08:49 |
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