Gustmann, Henrik ; Segler, Anna-Lena J. ; Gophane, Dnyaneshwar B. ; Reuss, Andreas J. ; Grünewald, Christian ; Braun, Markus ; Weigand, Julia E. ; Sigurdsson, Snorri Th. ; Wachtveitl, Josef (2019)
Structure guided fluorescence labeling reveals a two-step binding mechanism of neomycin to its RNA aptamer.
In: Nucleic acids research, 47 (1)
doi: 10.1093/nar/gky1110
Artikel, Bibliographie
Kurzbeschreibung (Abstract)
The ability of the cytidine analog Çmf to act as a position specific reporter of RNA-dynamics was spectroscopically evaluated. Çmf-labeled single- and double-stranded RNAs differ in their fluorescence lifetimes, quantum yields and anisotropies. These observables were also influenced by the nucleobases flanking Çmf. This conformation and position specificity allowed to investigate the binding dynamics and mechanism of neomycin to its aptamer N1 by independently incorporating Çmf at four different positions within the aptamer. Remarkably fast binding kinetics of neomycin binding was observed with stopped-flow measurements, which could be satisfactorily explained with a two-step binding. Conformational selection was identified as the dominant mechanism.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2019 |
| Autor(en): | Gustmann, Henrik ; Segler, Anna-Lena J. ; Gophane, Dnyaneshwar B. ; Reuss, Andreas J. ; Grünewald, Christian ; Braun, Markus ; Weigand, Julia E. ; Sigurdsson, Snorri Th. ; Wachtveitl, Josef |
| Art des Eintrags: | Bibliographie |
| Titel: | Structure guided fluorescence labeling reveals a two-step binding mechanism of neomycin to its RNA aptamer |
| Sprache: | Englisch |
| Publikationsjahr: | 10 Januar 2019 |
| Verlag: | Oxford University Press |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Nucleic acids research |
| Jahrgang/Volume einer Zeitschrift: | 47 |
| (Heft-)Nummer: | 1 |
| DOI: | 10.1093/nar/gky1110 |
| URL / URN: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6326822/ |
| Kurzbeschreibung (Abstract): | The ability of the cytidine analog Çmf to act as a position specific reporter of RNA-dynamics was spectroscopically evaluated. Çmf-labeled single- and double-stranded RNAs differ in their fluorescence lifetimes, quantum yields and anisotropies. These observables were also influenced by the nucleobases flanking Çmf. This conformation and position specificity allowed to investigate the binding dynamics and mechanism of neomycin to its aptamer N1 by independently incorporating Çmf at four different positions within the aptamer. Remarkably fast binding kinetics of neomycin binding was observed with stopped-flow measurements, which could be satisfactorily explained with a two-step binding. Conformational selection was identified as the dominant mechanism. |
| ID-Nummer: | pmid:30462266 |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie 10 Fachbereich Biologie > RNA Biochemie |
| Hinterlegungsdatum: | 05 Mär 2021 07:57 |
| Letzte Änderung: | 05 Mär 2021 07:57 |
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