Kemmerer, Katrin ; Weigand, Julia E. (2014)
Hypoxia reduces MAX expression in endothelial cells by unproductive splicing.
In: FEBS letters, 588 (24)
doi: 10.1016/j.febslet.2014.11.011
Artikel, Bibliographie
Kurzbeschreibung (Abstract)
The MYC-MAX-MXD network is involved in the regulation of cell differentiation and proliferation. Hypoxia affects the expression levels of several members of this network, but changes specific to MAX expression have so far not been shown. We found that in endothelial cells, hypoxia induces alternative splicing of MAX, thereby increasing the expression of two MAX isoforms that differ from the wild type in their 3' end. Isoform C is degraded by nonsense-mediated decay and isoform E encodes a highly unstable protein. The instability of isoform E is conferred by 36 isoform-specific amino acids, which have the capacity to destabilize heterologous proteins. Both splicing events are therefore unproductive and serve the purpose to downregulate the wild type protein.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2014 |
| Autor(en): | Kemmerer, Katrin ; Weigand, Julia E. |
| Art des Eintrags: | Bibliographie |
| Titel: | Hypoxia reduces MAX expression in endothelial cells by unproductive splicing |
| Sprache: | Englisch |
| Publikationsjahr: | 15 November 2014 |
| Verlag: | Wiley |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | FEBS letters |
| Jahrgang/Volume einer Zeitschrift: | 588 |
| (Heft-)Nummer: | 24 |
| DOI: | 10.1016/j.febslet.2014.11.011 |
| URL / URN: | https://febs.onlinelibrary.wiley.com/doi/10.1016/j.febslet.2... |
| Kurzbeschreibung (Abstract): | The MYC-MAX-MXD network is involved in the regulation of cell differentiation and proliferation. Hypoxia affects the expression levels of several members of this network, but changes specific to MAX expression have so far not been shown. We found that in endothelial cells, hypoxia induces alternative splicing of MAX, thereby increasing the expression of two MAX isoforms that differ from the wild type in their 3' end. Isoform C is degraded by nonsense-mediated decay and isoform E encodes a highly unstable protein. The instability of isoform E is conferred by 36 isoform-specific amino acids, which have the capacity to destabilize heterologous proteins. Both splicing events are therefore unproductive and serve the purpose to downregulate the wild type protein. |
| ID-Nummer: | pmid:25451222 |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie 10 Fachbereich Biologie > RNA Biochemie |
| Hinterlegungsdatum: | 05 Mär 2021 07:31 |
| Letzte Änderung: | 05 Mär 2021 07:31 |
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