Kukovetz, Kerri ; Hertel, Brigitte ; Schvarcz, Christopher R. ; Saponaro, Andrea ; Manthey, Mirja ; Burk, Ulrike ; Greiner, Timo ; Steward, Grieg F. ; Etten, James L. van ; Moroni, Anna ; Thiel, Gerhard ; Rauh, Oliver (2020)
A Functional K+ Channel from Tetraselmis Virus 1, a Member of the Mimiviridae.
In: Viruses, 12 (10)
doi: 10.3390/v12101107
Artikel, Bibliographie
Dies ist die neueste Version dieses Eintrags.
Kurzbeschreibung (Abstract)
Potassium ion (K) channels have been observed in diverse viruses that infect eukaryotic marine and freshwater algae. However, experimental evidence for functional K channels among these alga-infecting viruses has thus far been restricted to members of the family which are large, double-stranded DNA viruses within the phylum . Recent sequencing projects revealed that alga-infecting members of , another family within this phylum, may also contain genes encoding K channels. Here we examine the structural features and the functional properties of putative K channels from four cultivated members of . While all four proteins contain variations of the conserved selectivity filter sequence of K channels, structural prediction algorithms suggest that only two of them have the required number and position of two transmembrane domains that are present in all K channels. After in vitro translation and reconstitution of the four proteins in planar lipid bilayers, we confirmed that one of them, a 79 amino acid protein from the virus Tetraselmis virus 1 (TetV-1), forms a functional ion channel with a distinct selectivity for K over Na and a sensitivity to Ba. Thus, virus-encoded K channels are not limited to but also occur in the members of . The large sequence diversity among the viral K channels implies multiple events of lateral gene transfer.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2020 |
| Autor(en): | Kukovetz, Kerri ; Hertel, Brigitte ; Schvarcz, Christopher R. ; Saponaro, Andrea ; Manthey, Mirja ; Burk, Ulrike ; Greiner, Timo ; Steward, Grieg F. ; Etten, James L. van ; Moroni, Anna ; Thiel, Gerhard ; Rauh, Oliver |
| Art des Eintrags: | Bibliographie |
| Titel: | A Functional K+ Channel from Tetraselmis Virus 1, a Member of the Mimiviridae. |
| Sprache: | Englisch |
| Publikationsjahr: | 29 September 2020 |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Viruses |
| Jahrgang/Volume einer Zeitschrift: | 12 |
| (Heft-)Nummer: | 10 |
| DOI: | 10.3390/v12101107 |
| Zugehörige Links: | |
| Kurzbeschreibung (Abstract): | Potassium ion (K) channels have been observed in diverse viruses that infect eukaryotic marine and freshwater algae. However, experimental evidence for functional K channels among these alga-infecting viruses has thus far been restricted to members of the family which are large, double-stranded DNA viruses within the phylum . Recent sequencing projects revealed that alga-infecting members of , another family within this phylum, may also contain genes encoding K channels. Here we examine the structural features and the functional properties of putative K channels from four cultivated members of . While all four proteins contain variations of the conserved selectivity filter sequence of K channels, structural prediction algorithms suggest that only two of them have the required number and position of two transmembrane domains that are present in all K channels. After in vitro translation and reconstitution of the four proteins in planar lipid bilayers, we confirmed that one of them, a 79 amino acid protein from the virus Tetraselmis virus 1 (TetV-1), forms a functional ion channel with a distinct selectivity for K over Na and a sensitivity to Ba. Thus, virus-encoded K channels are not limited to but also occur in the members of . The large sequence diversity among the viral K channels implies multiple events of lateral gene transfer. |
| ID-Nummer: | pmid:33003637 |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie 10 Fachbereich Biologie > Plant Membrane Biophyscis (am 20.12.23 umbenannt in Biologie der Algen und Protozoen) |
| Hinterlegungsdatum: | 12 Okt 2020 09:51 |
| Letzte Änderung: | 03 Jul 2024 02:47 |
| PPN: | |
| Export: | |
| Suche nach Titel in: | TUfind oder in Google |
Verfügbare Versionen dieses Eintrags
-
A Functional K+ Channel from Tetraselmis Virus 1, a Member of the Mimiviridae. (deposited 03 Aug 2021 07:28)
- A Functional K+ Channel from Tetraselmis Virus 1, a Member of the Mimiviridae. (deposited 12 Okt 2020 09:51) [Gegenwärtig angezeigt]
 |
Frage zum Eintrag |
Optionen (nur für Redakteure)
 |
Redaktionelle Details anzeigen |
Drucken
Impressum