Hildebrand, Sven ; Schueth, Anna ; Wangenheim, Klaus von ; Mattheyer, Christian ; Pampaloni, Francesco ; Bratzke, Hansjürgen ; Roebroeck, Alard F. ; Galuske, Ralf A. W. (2020)
hFRUIT: An optimized agent for optical clearing of DiI-stained adult human brain tissue.
In: Scientific reports, 10 (1)
doi: 10.1038/s41598-020-66999-3
Artikel, Bibliographie
Kurzbeschreibung (Abstract)
Here, we describe a new immersion-based clearing method suitable for optical clearing of thick adult human brain samples while preserving its lipids and lipophilic labels such as 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (DiI). This clearing procedure is simple, easy to implement, and allowed for clearing of 5 mm thick human brain tissue samples within 12 days. Furthermore, we show for the first time the advantageous effect of the Periodate-Lysine-Paraformaldehyde (PLP) fixation as compared to the more commonly used 4% paraformaldehyde (PFA) on clearing performance.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2020 |
| Autor(en): | Hildebrand, Sven ; Schueth, Anna ; Wangenheim, Klaus von ; Mattheyer, Christian ; Pampaloni, Francesco ; Bratzke, Hansjürgen ; Roebroeck, Alard F. ; Galuske, Ralf A. W. |
| Art des Eintrags: | Bibliographie |
| Titel: | hFRUIT: An optimized agent for optical clearing of DiI-stained adult human brain tissue |
| Sprache: | Englisch |
| Publikationsjahr: | 19 Juni 2020 |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Scientific reports |
| Jahrgang/Volume einer Zeitschrift: | 10 |
| (Heft-)Nummer: | 1 |
| DOI: | 10.1038/s41598-020-66999-3 |
| Kurzbeschreibung (Abstract): | Here, we describe a new immersion-based clearing method suitable for optical clearing of thick adult human brain samples while preserving its lipids and lipophilic labels such as 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (DiI). This clearing procedure is simple, easy to implement, and allowed for clearing of 5 mm thick human brain tissue samples within 12 days. Furthermore, we show for the first time the advantageous effect of the Periodate-Lysine-Paraformaldehyde (PLP) fixation as compared to the more commonly used 4% paraformaldehyde (PFA) on clearing performance. |
| ID-Nummer: | pmid:32561795 |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie 10 Fachbereich Biologie > Systemische Neurophysiologie |
| Hinterlegungsdatum: | 25 Jun 2020 05:38 |
| Letzte Änderung: | 03 Feb 2022 08:19 |
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