Müller, Lena K. ; Duznovic, Ivana ; Tietze, Daniel ; Weber, Wadim ; Ali, Mubarak ; Stein, Viktor ; Ensinger, Wolfgang ; Tietze, Alesia A. (2020)
Ultrasensitive and selective copper(II) detection: introducing a bioinspired and robust sensor.
In: Chemistry – A European Journal, 26 (39)
doi: 10.1002/chem.202001160
Artikel, Bibliographie
Kurzbeschreibung (Abstract)
We report a nanopore-based Cu(II) sensing system which allows for an ultrasensitive and selective detection of Cu(II) with the possibility for a broad range of applications, i.e. medical diagnostics. A fluorescent ATCUN-like peptide 5/6-FAM-Dap-β-Ala-His is employed to selectively bind Cu(II) ions in the presence of Ni(II) and Zn(II) and was crafted into ion track-etched nanopores. Upon Cu(II) binding the fluorescence of the peptide sensor is quenched, permitting the detection of Cu(II) in solution. The ion transport characteristics of peptide-modified nanopore are shown to be extremely sensitive and selective towards Cu(II) allowing to sense femtomolar Cu(II) concentrations in human urine mimics. Washing with EDTA fully restores the Cu(II)-binding properties of the sensor, enabling multiple repetitive measurements. The robustness of the system clearly has the potential to be further developed into an easy-to-use, lab-on-chip Cu(II)-sensing device, which will be of great importance for bedside diagnosis and monitor of Cu(II) levels in patients with copper dysfunctional homeostasis.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2020 |
| Autor(en): | Müller, Lena K. ; Duznovic, Ivana ; Tietze, Daniel ; Weber, Wadim ; Ali, Mubarak ; Stein, Viktor ; Ensinger, Wolfgang ; Tietze, Alesia A. |
| Art des Eintrags: | Bibliographie |
| Titel: | Ultrasensitive and selective copper(II) detection: introducing a bioinspired and robust sensor |
| Sprache: | Englisch |
| Publikationsjahr: | 14 Juli 2020 |
| Verlag: | Wiley-VCH |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Chemistry – A European Journal |
| Jahrgang/Volume einer Zeitschrift: | 26 |
| (Heft-)Nummer: | 39 |
| DOI: | 10.1002/chem.202001160 |
| Kurzbeschreibung (Abstract): | We report a nanopore-based Cu(II) sensing system which allows for an ultrasensitive and selective detection of Cu(II) with the possibility for a broad range of applications, i.e. medical diagnostics. A fluorescent ATCUN-like peptide 5/6-FAM-Dap-β-Ala-His is employed to selectively bind Cu(II) ions in the presence of Ni(II) and Zn(II) and was crafted into ion track-etched nanopores. Upon Cu(II) binding the fluorescence of the peptide sensor is quenched, permitting the detection of Cu(II) in solution. The ion transport characteristics of peptide-modified nanopore are shown to be extremely sensitive and selective towards Cu(II) allowing to sense femtomolar Cu(II) concentrations in human urine mimics. Washing with EDTA fully restores the Cu(II)-binding properties of the sensor, enabling multiple repetitive measurements. The robustness of the system clearly has the potential to be further developed into an easy-to-use, lab-on-chip Cu(II)-sensing device, which will be of great importance for bedside diagnosis and monitor of Cu(II) levels in patients with copper dysfunctional homeostasis. |
| Freie Schlagworte: | Liebig Fellowship of the Fonds der Chemischen Industrie, LOEWE project iNAPO - Ministry of Higher education, Research and the Arts (HMWK) of the Hessen state, Knut & Alice Wallenberg Foundation, ATCUN, copper, fluorescence, nanopores, sensors |
| ID-Nummer: | pmid:32196774 |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie 10 Fachbereich Biologie > Protein Engineering of Ion Conducting Nanopores 11 Fachbereich Material- und Geowissenschaften 11 Fachbereich Material- und Geowissenschaften > Materialwissenschaft 11 Fachbereich Material- und Geowissenschaften > Materialwissenschaft > Fachgebiet Materialanalytik |
| Hinterlegungsdatum: | 25 Mär 2020 07:46 |
| Letzte Änderung: | 18 Nov 2020 13:41 |
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