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Biomolecular Detection with a Single Nanofluidic Diode Decorated with Metal Chelates

Ali, Mubarak and Nasir, Saima and Ahmed, Ishtiaq and Niemeyer, Christof M. and Ensinger, Wolfgang (2020):
Biomolecular Detection with a Single Nanofluidic Diode Decorated with Metal Chelates.
In: ChemPlusChem, Wiley VCH, ISSN 2192-6506,
DOI: 10.1002/cplu.202000045,
[Online-Edition: https://doi.org/10.1002/cplu.202000045],
[Article]

Abstract

Here we demonstrate a nanofluidic device for the label‐free detection of phosphoprotein (PPn) analytes. To achieve this goal, a metal ion chelator, i.e., the 4‐[bis(2‐pyridylmethyl)aminomethyl]aniline (DPA–NH2) compound is synthesized. Single asymmetric nanofluidic channels are fabricated in polyethylene terephthalate (PET) membranes. Then the chelator (DPA–NH2) molecules are immobilized on the nanochannel surface followed by the zinc ion complexation to afford DPA–Zn2+ chelates which act as ligand moieties for the specific binding of phosphoproteins. The success of chemical reaction and biomolecular recognition process occurring in confined geometry is monitored from the changes in electrical readout of the nanochannel. The designed nanofluidic sensor has the ability to sensitively and specifically detect lower concentrations (≥1 nM) of phosphoprotein (albumin and α‐casein) in the surrounding environment as evidenced from the significant decrease in ion current flowing through the nanochannels. While, dephosphoproteins such as lysozyme and dephospho‐α‐casein even at higher concentration (˃1 µM) could not induce any significant change in the transmembrane ion flux. This indicated the sensitivity and specificity of the proposed nanofluidic sensor towards PPn proteins. In this context, we believe that metal affinity‐based nanofluidic sensor would readily be used to differentiate in between phosphoproteins and dephosphoproteins

Item Type: Article
Erschienen: 2020
Creators: Ali, Mubarak and Nasir, Saima and Ahmed, Ishtiaq and Niemeyer, Christof M. and Ensinger, Wolfgang
Title: Biomolecular Detection with a Single Nanofluidic Diode Decorated with Metal Chelates
Language: English
Abstract:

Here we demonstrate a nanofluidic device for the label‐free detection of phosphoprotein (PPn) analytes. To achieve this goal, a metal ion chelator, i.e., the 4‐[bis(2‐pyridylmethyl)aminomethyl]aniline (DPA–NH2) compound is synthesized. Single asymmetric nanofluidic channels are fabricated in polyethylene terephthalate (PET) membranes. Then the chelator (DPA–NH2) molecules are immobilized on the nanochannel surface followed by the zinc ion complexation to afford DPA–Zn2+ chelates which act as ligand moieties for the specific binding of phosphoproteins. The success of chemical reaction and biomolecular recognition process occurring in confined geometry is monitored from the changes in electrical readout of the nanochannel. The designed nanofluidic sensor has the ability to sensitively and specifically detect lower concentrations (≥1 nM) of phosphoprotein (albumin and α‐casein) in the surrounding environment as evidenced from the significant decrease in ion current flowing through the nanochannels. While, dephosphoproteins such as lysozyme and dephospho‐α‐casein even at higher concentration (˃1 µM) could not induce any significant change in the transmembrane ion flux. This indicated the sensitivity and specificity of the proposed nanofluidic sensor towards PPn proteins. In this context, we believe that metal affinity‐based nanofluidic sensor would readily be used to differentiate in between phosphoproteins and dephosphoproteins

Journal or Publication Title: ChemPlusChem
Publisher: Wiley VCH
Uncontrolled Keywords: biomolecules, ion current rectification, ligand-receptor interactions, sensors, synthetic nanochannels
Divisions: 11 Department of Materials and Earth Sciences
11 Department of Materials and Earth Sciences > Material Science
11 Department of Materials and Earth Sciences > Material Science > Material Analytics
Date Deposited: 25 Mar 2020 06:43
DOI: 10.1002/cplu.202000045
Official URL: https://doi.org/10.1002/cplu.202000045
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