Yue, Ke ; Tran, Nam Trung ; Zhu, Yiyong ; Kaldenhoff, Ralf ; Kai, Lei (2019)
Co-Translational Insertion of Aquaporins into Liposome for Functional Analysis via an E. coli Based Cell-Free Protein Synthesis System.
In: Cells, 8 (11)
doi: 10.3390/cells8111325
Artikel, Bibliographie
Dies ist die neueste Version dieses Eintrags.
Kurzbeschreibung (Abstract)
Aquaporins are important and well-studied water channel membrane proteins. However, being membrane proteins, sample preparation for functional analysis is tedious and time-consuming. In this paper, we report a new approach for the co-translational insertion of two aquaporins from and using the CFPS system. This was done in the presence of liposomes with a modified procedure to form homogenous proteo-liposomes suitable for functional analysis of water permeability using stopped-flow spectrophotometry. Two model aquaporins, AqpZ and PIP2;1, were successfully incorporated into the liposome in their active forms. Shifted green fluorescent protein was fused to the C-terminal part of AqpZ to monitor its insertion and status in the lipid environment. This new fast approach offers a fast and straightforward method for the functional analysis of aquaporins in both prokaryotic and eukaryotic organisms.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2019 |
| Autor(en): | Yue, Ke ; Tran, Nam Trung ; Zhu, Yiyong ; Kaldenhoff, Ralf ; Kai, Lei |
| Art des Eintrags: | Bibliographie |
| Titel: | Co-Translational Insertion of Aquaporins into Liposome for Functional Analysis via an E. coli Based Cell-Free Protein Synthesis System. |
| Sprache: | Englisch |
| Publikationsjahr: | 27 Oktober 2019 |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Cells |
| Jahrgang/Volume einer Zeitschrift: | 8 |
| (Heft-)Nummer: | 11 |
| DOI: | 10.3390/cells8111325 |
| Zugehörige Links: | |
| Kurzbeschreibung (Abstract): | Aquaporins are important and well-studied water channel membrane proteins. However, being membrane proteins, sample preparation for functional analysis is tedious and time-consuming. In this paper, we report a new approach for the co-translational insertion of two aquaporins from and using the CFPS system. This was done in the presence of liposomes with a modified procedure to form homogenous proteo-liposomes suitable for functional analysis of water permeability using stopped-flow spectrophotometry. Two model aquaporins, AqpZ and PIP2;1, were successfully incorporated into the liposome in their active forms. Shifted green fluorescent protein was fused to the C-terminal part of AqpZ to monitor its insertion and status in the lipid environment. This new fast approach offers a fast and straightforward method for the functional analysis of aquaporins in both prokaryotic and eukaryotic organisms. |
| ID-Nummer: | pmid:31717877 |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie 10 Fachbereich Biologie > Applied Plant Sciences |
| Hinterlegungsdatum: | 18 Nov 2019 11:42 |
| Letzte Änderung: | 04 Dez 2023 12:23 |
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| Suche nach Titel in: | TUfind oder in Google |
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Co-Translational Insertion of Aquaporins into Liposome for Functional Analysis via an E. coli Based Cell-Free Protein Synthesis System. (deposited 01 Dez 2023 14:07)
- Co-Translational Insertion of Aquaporins into Liposome for Functional Analysis via an E. coli Based Cell-Free Protein Synthesis System. (deposited 18 Nov 2019 11:42) [Gegenwärtig angezeigt]
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