Bandmann, Vera ; Mirsanaye, Ann Schirin ; Schäfer, Johanna ; Thiel, Gerhard ; Holstein, Thomas ; Mikosch-Wersching, Melanie (2019)
Membrane capacitance recordings resolve dynamics and complexity of receptor-mediated endocytosis in Wnt signalling.
In: Scientific Reports, 2019, 9 (1)
doi: 10.1038/s41598-019-49082-4
Artikel, Zweitveröffentlichung, Verlagsversion
 | Es ist eine neuere Version dieses Eintrags verfügbar. |
Kurzbeschreibung (Abstract)
Receptor-mediated endocytosis is an essential process in signalling pathways for activation of intracellular signalling cascades. One example is the Wnt signalling pathway that seems to depend on endocytosis of the ligand-receptor complex for initiation of Wnt signal transduction. To date, the roles of diferent endocytic pathways in Wnt signalling, molecular players and the kinetics of the process remain unclear. Here, we monitored endocytosis in Wnt3a and Wnt5a-mediated signalling with membrane capacitance recordings of HEK293 cells. Our measurements revealed a swift and substantial increase in the number of endocytic vesicles. Extracellular Wnt ligands specifcally triggered endocytotic activity, which started immediately upon ligand binding and ceased within a period of ten minutes. By using specifc inhibitors, we were able to separate Wnt-induced endocytosis into two independent pathways. We demonstrate that canonical Wnt3a is taken up mainly by clathrin-independent endocytosis whereas noncanonical Wnt5a is exclusively regulated via clathrin-mediated endocytosis. Our fndings show that membrane capacitance recordings allow the resolution of complex cellular processes in plasma membrane signalling pathways in great detail.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2019 |
| Autor(en): | Bandmann, Vera ; Mirsanaye, Ann Schirin ; Schäfer, Johanna ; Thiel, Gerhard ; Holstein, Thomas ; Mikosch-Wersching, Melanie |
| Art des Eintrags: | Zweitveröffentlichung |
| Titel: | Membrane capacitance recordings resolve dynamics and complexity of receptor-mediated endocytosis in Wnt signalling |
| Sprache: | Englisch |
| Publikationsjahr: | 2019 |
| Publikationsdatum der Erstveröffentlichung: | 2019 |
| Verlag: | Springer Nature |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Scientific Reports |
| Jahrgang/Volume einer Zeitschrift: | 9 |
| (Heft-)Nummer: | 1 |
| DOI: | 10.1038/s41598-019-49082-4 |
| URL / URN: | https://doi.org/10.1038/s41598-019-49082-4 |
| Herkunft: | Zweitveröffentlichung aus gefördertem Golden Open Access |
| Kurzbeschreibung (Abstract): | Receptor-mediated endocytosis is an essential process in signalling pathways for activation of intracellular signalling cascades. One example is the Wnt signalling pathway that seems to depend on endocytosis of the ligand-receptor complex for initiation of Wnt signal transduction. To date, the roles of diferent endocytic pathways in Wnt signalling, molecular players and the kinetics of the process remain unclear. Here, we monitored endocytosis in Wnt3a and Wnt5a-mediated signalling with membrane capacitance recordings of HEK293 cells. Our measurements revealed a swift and substantial increase in the number of endocytic vesicles. Extracellular Wnt ligands specifcally triggered endocytotic activity, which started immediately upon ligand binding and ceased within a period of ten minutes. By using specifc inhibitors, we were able to separate Wnt-induced endocytosis into two independent pathways. We demonstrate that canonical Wnt3a is taken up mainly by clathrin-independent endocytosis whereas noncanonical Wnt5a is exclusively regulated via clathrin-mediated endocytosis. Our fndings show that membrane capacitance recordings allow the resolution of complex cellular processes in plasma membrane signalling pathways in great detail. |
| Status: | Verlagsversion |
| URN: | urn:nbn:de:tuda-tuprints-92218 |
| Sachgruppe der Dewey Dezimalklassifikatin (DDC): | 500 Naturwissenschaften und Mathematik > 570 Biowissenschaften, Biologie |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie 10 Fachbereich Biologie > Plant Membrane Biophyscis (am 20.12.23 umbenannt in Biologie der Algen und Protozoen) |
| Hinterlegungsdatum: | 03 Nov 2019 20:57 |
| Letzte Änderung: | 03 Nov 2019 20:57 |
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| Suche nach Titel in: | TUfind oder in Google |
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- Membrane capacitance recordings resolve dynamics and complexity of receptor-mediated endocytosis in Wnt signalling. (deposited 03 Nov 2019 20:57) [Gegenwärtig angezeigt]
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