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Lipid determinants of endocytosis and exocytosis in budding yeast.

Degreif, Daniel and Cucu, Bayram and Budin, Itay and Thiel, Gerhard and Bertl, Adam (2019):
Lipid determinants of endocytosis and exocytosis in budding yeast.
In: Biochimica et biophysica acta. Molecular and cell biology of lipids, 1864 (7), pp. 1005-1016. ISSN 1879-2618,
DOI: 10.1016/j.bbalip.2019.03.007,
[Article]

Abstract

Cells maintain physicochemical characteristics of membranes in order to allow for proper function of membrane-associated cellular processes, such as endocytosis and exocytosis. To investigate the interplay between membrane properties and biological processes, we applied lipid engineering approaches that allowed for systematic manipulation of fatty acid unsaturation and sterol biosynthesis, the main regulators of membrane fluidity. In combination with electrophysiological membrane capacitance measurements, we were able to study the dependence of the endo- and exocytic activity of Saccharomyces cerevisiae on membrane lipid composition in vivo. We found that a strong decrease in the cell's total ergosterol content leads to a severely reduced frequency of vesicle fission (endocytosis), whereas the exocytic activity remained largely unaffected. In contrast, increased lipid saturation lowered both endocytic and the exocytic activity, with the former being more severely affected. We were able to correlate the decreased ratio of endocytic/exocytic frequencies (f/f) upon lipid perturbation with the growth of yeast protoplasts, which is based on a surface enlargement resulting from a net excess of exocytic over endocytic flux. Experiments using clathrin-deficient mutants confirm a correlation between reduced endocytic activity and increased size of intact walled cells, as well as accelerated protoplast growth. These data show that lipid composition is intimately tied to membrane trafficking in yeast cells and suggest that endocytosis is particularly dependent on the lipid-defined properties of cell membrane.

Item Type: Article
Erschienen: 2019
Creators: Degreif, Daniel and Cucu, Bayram and Budin, Itay and Thiel, Gerhard and Bertl, Adam
Title: Lipid determinants of endocytosis and exocytosis in budding yeast.
Language: English
Abstract:

Cells maintain physicochemical characteristics of membranes in order to allow for proper function of membrane-associated cellular processes, such as endocytosis and exocytosis. To investigate the interplay between membrane properties and biological processes, we applied lipid engineering approaches that allowed for systematic manipulation of fatty acid unsaturation and sterol biosynthesis, the main regulators of membrane fluidity. In combination with electrophysiological membrane capacitance measurements, we were able to study the dependence of the endo- and exocytic activity of Saccharomyces cerevisiae on membrane lipid composition in vivo. We found that a strong decrease in the cell's total ergosterol content leads to a severely reduced frequency of vesicle fission (endocytosis), whereas the exocytic activity remained largely unaffected. In contrast, increased lipid saturation lowered both endocytic and the exocytic activity, with the former being more severely affected. We were able to correlate the decreased ratio of endocytic/exocytic frequencies (f/f) upon lipid perturbation with the growth of yeast protoplasts, which is based on a surface enlargement resulting from a net excess of exocytic over endocytic flux. Experiments using clathrin-deficient mutants confirm a correlation between reduced endocytic activity and increased size of intact walled cells, as well as accelerated protoplast growth. These data show that lipid composition is intimately tied to membrane trafficking in yeast cells and suggest that endocytosis is particularly dependent on the lipid-defined properties of cell membrane.

Journal or Publication Title: Biochimica et biophysica acta. Molecular and cell biology of lipids
Journal volume: 1864
Number: 7
Divisions: 10 Department of Biology
10 Department of Biology > Yeast Membrane Biology
10 Department of Biology > Plant Membrane Biophysics
Date Deposited: 15 Apr 2019 09:42
DOI: 10.1016/j.bbalip.2019.03.007
Identification Number: pmid:30917917
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