Degreif, Daniel ; Cucu, Bayram ; Budin, Itay ; Thiel, Gerhard ; Bertl, Adam (2019)
Lipid determinants of endocytosis and exocytosis in budding yeast.
In: Biochimica et biophysica acta. Molecular and cell biology of lipids, 1864 (7)
doi: 10.1016/j.bbalip.2019.03.007
Artikel, Bibliographie
Kurzbeschreibung (Abstract)
Cells maintain physicochemical characteristics of membranes in order to allow for proper function of membrane-associated cellular processes, such as endocytosis and exocytosis. To investigate the interplay between membrane properties and biological processes, we applied lipid engineering approaches that allowed for systematic manipulation of fatty acid unsaturation and sterol biosynthesis, the main regulators of membrane fluidity. In combination with electrophysiological membrane capacitance measurements, we were able to study the dependence of the endo- and exocytic activity of Saccharomyces cerevisiae on membrane lipid composition in vivo. We found that a strong decrease in the cell's total ergosterol content leads to a severely reduced frequency of vesicle fission (endocytosis), whereas the exocytic activity remained largely unaffected. In contrast, increased lipid saturation lowered both endocytic and the exocytic activity, with the former being more severely affected. We were able to correlate the decreased ratio of endocytic/exocytic frequencies (f/f) upon lipid perturbation with the growth of yeast protoplasts, which is based on a surface enlargement resulting from a net excess of exocytic over endocytic flux. Experiments using clathrin-deficient mutants confirm a correlation between reduced endocytic activity and increased size of intact walled cells, as well as accelerated protoplast growth. These data show that lipid composition is intimately tied to membrane trafficking in yeast cells and suggest that endocytosis is particularly dependent on the lipid-defined properties of cell membrane.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2019 |
| Autor(en): | Degreif, Daniel ; Cucu, Bayram ; Budin, Itay ; Thiel, Gerhard ; Bertl, Adam |
| Art des Eintrags: | Bibliographie |
| Titel: | Lipid determinants of endocytosis and exocytosis in budding yeast. |
| Sprache: | Englisch |
| Publikationsjahr: | 24 März 2019 |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Biochimica et biophysica acta. Molecular and cell biology of lipids |
| Jahrgang/Volume einer Zeitschrift: | 1864 |
| (Heft-)Nummer: | 7 |
| DOI: | 10.1016/j.bbalip.2019.03.007 |
| Kurzbeschreibung (Abstract): | Cells maintain physicochemical characteristics of membranes in order to allow for proper function of membrane-associated cellular processes, such as endocytosis and exocytosis. To investigate the interplay between membrane properties and biological processes, we applied lipid engineering approaches that allowed for systematic manipulation of fatty acid unsaturation and sterol biosynthesis, the main regulators of membrane fluidity. In combination with electrophysiological membrane capacitance measurements, we were able to study the dependence of the endo- and exocytic activity of Saccharomyces cerevisiae on membrane lipid composition in vivo. We found that a strong decrease in the cell's total ergosterol content leads to a severely reduced frequency of vesicle fission (endocytosis), whereas the exocytic activity remained largely unaffected. In contrast, increased lipid saturation lowered both endocytic and the exocytic activity, with the former being more severely affected. We were able to correlate the decreased ratio of endocytic/exocytic frequencies (f/f) upon lipid perturbation with the growth of yeast protoplasts, which is based on a surface enlargement resulting from a net excess of exocytic over endocytic flux. Experiments using clathrin-deficient mutants confirm a correlation between reduced endocytic activity and increased size of intact walled cells, as well as accelerated protoplast growth. These data show that lipid composition is intimately tied to membrane trafficking in yeast cells and suggest that endocytosis is particularly dependent on the lipid-defined properties of cell membrane. |
| ID-Nummer: | pmid:30917917 |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie 10 Fachbereich Biologie > Yeast Membrane Biology 10 Fachbereich Biologie > Plant Membrane Biophyscis (am 20.12.23 umbenannt in Biologie der Algen und Protozoen) |
| Hinterlegungsdatum: | 15 Apr 2019 09:42 |
| Letzte Änderung: | 15 Apr 2019 09:42 |
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