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Atomic force microscopy measurements probing the mechanical properties of single collagen fibrils under the influence of UV light in situ

Schulze, Marcus and Rogge, Melanie and Stark, Robert W. (2018):
Atomic force microscopy measurements probing the mechanical properties of single collagen fibrils under the influence of UV light in situ.
In: Journal of the Mechanical Behavior of Biomedical Materials, Elsevier Science Publishing, pp. 415-421, (88), ISSN 17516161, DOI: 10.1016/j.jmbbm.2018.08.039, [Online-Edition: https://doi.org/10.1016/j.jmbbm.2018.08.039],
[Article]

Abstract

Collagen plays a decisive role as a functional substrate in tissue engineering. In particular, the rigidity of the collagen influences the behaviour of the attached cells. Thus, modification and controlled adjustment of collagen's characteristics are essential. To this end, controlled exposure to ultraviolet (UV) light is a promising process because it can be temporally and spatially well defined. In this study, we investigated the effect of UV exposure on surface supported single collagen fibrils in situ. This procedure allowed for a direct comparison between the untreated and modified states of type I collagen. Atomic force microscopy was used to map the mechanical properties. Exposure to UV light was used to influence the mechanical properties of the fibrils in varied liquid environments (deionized water and phosphate-buffered saline (PBS)). The results led to the assumption that combined UV/thermal treatment in deionized water continuously lowers the elastic modulus. In contrast, experiments performed in PBS-based solutions in combination with UV-B and UV-C light or thermal treatment up to 45 °C suggested an increase in the modulus within the first 30–40 min that subsequently decreased again. Thus, the wavelength, exposure, temperature, and chemical environment are relevant parameters that need to be controlled when modifying collagen using UV light.

Item Type: Article
Erschienen: 2018
Creators: Schulze, Marcus and Rogge, Melanie and Stark, Robert W.
Title: Atomic force microscopy measurements probing the mechanical properties of single collagen fibrils under the influence of UV light in situ
Language: English
Abstract:

Collagen plays a decisive role as a functional substrate in tissue engineering. In particular, the rigidity of the collagen influences the behaviour of the attached cells. Thus, modification and controlled adjustment of collagen's characteristics are essential. To this end, controlled exposure to ultraviolet (UV) light is a promising process because it can be temporally and spatially well defined. In this study, we investigated the effect of UV exposure on surface supported single collagen fibrils in situ. This procedure allowed for a direct comparison between the untreated and modified states of type I collagen. Atomic force microscopy was used to map the mechanical properties. Exposure to UV light was used to influence the mechanical properties of the fibrils in varied liquid environments (deionized water and phosphate-buffered saline (PBS)). The results led to the assumption that combined UV/thermal treatment in deionized water continuously lowers the elastic modulus. In contrast, experiments performed in PBS-based solutions in combination with UV-B and UV-C light or thermal treatment up to 45 °C suggested an increase in the modulus within the first 30–40 min that subsequently decreased again. Thus, the wavelength, exposure, temperature, and chemical environment are relevant parameters that need to be controlled when modifying collagen using UV light.

Journal or Publication Title: Journal of the Mechanical Behavior of Biomedical Materials
Number: 88
Publisher: Elsevier Science Publishing
Uncontrolled Keywords: AFM, Collagen fibril, UV, Thermal treatment, Modulus, In situ
Divisions: 11 Department of Materials and Earth Sciences
11 Department of Materials and Earth Sciences > Material Science
11 Department of Materials and Earth Sciences > Material Science > Physics of Surfaces
Date Deposited: 10 Sep 2018 08:52
DOI: 10.1016/j.jmbbm.2018.08.039
Official URL: https://doi.org/10.1016/j.jmbbm.2018.08.039
Funders: The authors would like to thank the Deutsche Forschungsgemeinschaft under grant STA 1026/7-1 for financial support.
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