Voos, Patrick ; Fuck, Sebastian ; Weipert, Fabian ; Babel, Laura ; Tandl, Dominique ; Meckel, Tobias ; Hehlgans, Stephanie ; Fournier, Claudia ; Moroni, Anna ; Rödel, Franz ; Thiel, Gerhard (2018)
Ionizing Radiation Induces Morphological Changes and Immunological Modulation of Jurkat Cells.
In: Frontiers in Immunology, 2018, 9
doi: 10.3389/fimmu.2018.00922
Artikel, Zweitveröffentlichung, Verlagsversion
 | Es ist eine neuere Version dieses Eintrags verfügbar. |
Kurzbeschreibung (Abstract)
Impairment or stimulation of the immune system by ionizing radiation (IR) impacts on immune surveillance of tumor cells and non-malignant cells and can either foster therapy response or side effects/toxicities of radiation therapy. For a better understanding of the mechanisms by which IR modulates T-cell activation and alters functional properties of these immune cells, we exposed human immortalized Jurkat cells and peripheral blood lymphocytes (PBL) to X-ray doses between 0.1 and 5 Gy. This resulted in cellular responses, which are typically observed also in naïve T-lymphocytes in response of T-cell receptor immune stimulation or mitogens. These responses include oscillations of cytosolic Ca2+, an upregulation of CD25 surface expression, interleukin-2 and interferon-γ synthesis, elevated expression of Ca2+ sensitive K+ channels and an increase in cell diameter. The latter was sensitive to inhibition by the immunosuppressant cyclosporine A, Ca2+ buffer BAPTA-AM, and the CDK1-inhibitor RO3306, indicating the involvement of Ca2+-dependent immune activation and radiation-induced cell cycle arrest. Furthermore, on a functional level, Jurkat and PBL cell adhesion to endothelial cells was increased upon radiation exposure and was highly dependent on an upregulation of integrin beta-1 expression and clustering. In conclusion, we here report that IR impacts on immune activation and functional properties of T-lymphocytes that may have implications in both toxic effects and treatment response to combined radiation and immune therapy in cancer patients.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2018 |
| Autor(en): | Voos, Patrick ; Fuck, Sebastian ; Weipert, Fabian ; Babel, Laura ; Tandl, Dominique ; Meckel, Tobias ; Hehlgans, Stephanie ; Fournier, Claudia ; Moroni, Anna ; Rödel, Franz ; Thiel, Gerhard |
| Art des Eintrags: | Zweitveröffentlichung |
| Titel: | Ionizing Radiation Induces Morphological Changes and Immunological Modulation of Jurkat Cells |
| Sprache: | Englisch |
| Publikationsjahr: | 2018 |
| Publikationsdatum der Erstveröffentlichung: | 2018 |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Frontiers in Immunology |
| Jahrgang/Volume einer Zeitschrift: | 9 |
| DOI: | 10.3389/fimmu.2018.00922 |
| URL / URN: | https://doi.org/10.3389/fimmu.2018.00922 |
| Herkunft: | Zweitveröffentlichung aus gefördertem Golden Open Access |
| Kurzbeschreibung (Abstract): | Impairment or stimulation of the immune system by ionizing radiation (IR) impacts on immune surveillance of tumor cells and non-malignant cells and can either foster therapy response or side effects/toxicities of radiation therapy. For a better understanding of the mechanisms by which IR modulates T-cell activation and alters functional properties of these immune cells, we exposed human immortalized Jurkat cells and peripheral blood lymphocytes (PBL) to X-ray doses between 0.1 and 5 Gy. This resulted in cellular responses, which are typically observed also in naïve T-lymphocytes in response of T-cell receptor immune stimulation or mitogens. These responses include oscillations of cytosolic Ca2+, an upregulation of CD25 surface expression, interleukin-2 and interferon-γ synthesis, elevated expression of Ca2+ sensitive K+ channels and an increase in cell diameter. The latter was sensitive to inhibition by the immunosuppressant cyclosporine A, Ca2+ buffer BAPTA-AM, and the CDK1-inhibitor RO3306, indicating the involvement of Ca2+-dependent immune activation and radiation-induced cell cycle arrest. Furthermore, on a functional level, Jurkat and PBL cell adhesion to endothelial cells was increased upon radiation exposure and was highly dependent on an upregulation of integrin beta-1 expression and clustering. In conclusion, we here report that IR impacts on immune activation and functional properties of T-lymphocytes that may have implications in both toxic effects and treatment response to combined radiation and immune therapy in cancer patients. |
| Status: | Verlagsversion |
| URN: | urn:nbn:de:tuda-tuprints-74493 |
| Sachgruppe der Dewey Dezimalklassifikatin (DDC): | 500 Naturwissenschaften und Mathematik > 570 Biowissenschaften, Biologie |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie 10 Fachbereich Biologie > Plant Membrane Biophyscis (am 20.12.23 umbenannt in Biologie der Algen und Protozoen) 10 Fachbereich Biologie > Membrane Dynamics |
| Hinterlegungsdatum: | 03 Jun 2018 19:55 |
| Letzte Änderung: | 05 Jul 2018 06:38 |
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| Suche nach Titel in: | TUfind oder in Google |
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- Ionizing Radiation Induces Morphological Changes and Immunological Modulation of Jurkat Cells. (deposited 03 Jun 2018 19:55) [Gegenwärtig angezeigt]
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