TU Darmstadt / ULB / TUbiblio

Directional K+ channel insertion in a single phospholipid bilayer: Neutron reflectometry and electrophysiology in the joint exploration of a model membrane functional platform

Rondelli, V. and Del Favero, E. and Brocca, P. and Fragneto, G. and Trapp, M. and Mauri, L. and Ciampa, M. G. and Romani, G. and Braun, C. J. and Winterstein, L. and Schroeder, Indra and Thiel, Gerhard and Moroni, Anna and Cantu', L. (2018):
Directional K+ channel insertion in a single phospholipid bilayer: Neutron reflectometry and electrophysiology in the joint exploration of a model membrane functional platform.
In: Biochimica et biophysica acta, pp. 1742-1750, 1862, (8), ISSN 0006-3002,
[Article]

Abstract

We investigated the insertion of small potassium (K) channel proteins (Kcv and Kcv) into model membranes and the lipid-protein structural interference, combining neutron reflectometry and electrophysiology. Neutron reflectometry experiments showed how the transverse structure and mechanical properties of the bilayer were modified, upon insertion of the proteins in single model-membranes, either supported on solid substrate or floating. Parallel electrophysiology experiments were performed on the same channels reconstituted in free-standing planar lipid bilayers, of both typical composition and matched to the neutron reflectometry experiment, assessing their electrical features. Functional and structural results converge in detecting that the proteins, conical in shape, insert with a directionality, cytosolic side first. Our work addresses the powerful combination of the two experimental approaches. We show here that membrane structure spectroscopy and ion channel electrophysiology can become synergistic tools in the analysis of structural-functional properties of biomimetic complex environment.

Item Type: Article
Erschienen: 2018
Creators: Rondelli, V. and Del Favero, E. and Brocca, P. and Fragneto, G. and Trapp, M. and Mauri, L. and Ciampa, M. G. and Romani, G. and Braun, C. J. and Winterstein, L. and Schroeder, Indra and Thiel, Gerhard and Moroni, Anna and Cantu', L.
Title: Directional K+ channel insertion in a single phospholipid bilayer: Neutron reflectometry and electrophysiology in the joint exploration of a model membrane functional platform
Language: English
Abstract:

We investigated the insertion of small potassium (K) channel proteins (Kcv and Kcv) into model membranes and the lipid-protein structural interference, combining neutron reflectometry and electrophysiology. Neutron reflectometry experiments showed how the transverse structure and mechanical properties of the bilayer were modified, upon insertion of the proteins in single model-membranes, either supported on solid substrate or floating. Parallel electrophysiology experiments were performed on the same channels reconstituted in free-standing planar lipid bilayers, of both typical composition and matched to the neutron reflectometry experiment, assessing their electrical features. Functional and structural results converge in detecting that the proteins, conical in shape, insert with a directionality, cytosolic side first. Our work addresses the powerful combination of the two experimental approaches. We show here that membrane structure spectroscopy and ion channel electrophysiology can become synergistic tools in the analysis of structural-functional properties of biomimetic complex environment.

Journal or Publication Title: Biochimica et biophysica acta
Volume: 1862
Number: 8
Divisions: 10 Department of Biology
10 Department of Biology > Plant Membrane Biophysics
Date Deposited: 14 May 2018 12:13
Identification Number: pmid:29753114
Export:
Suche nach Titel in: TUfind oder in Google

Optionen (nur für Redakteure)

View Item View Item