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Altered stoichiometry of an evolved RNA aptamer.

Ohuchi, Shoji ; Suess, Beatrix (2018):
Altered stoichiometry of an evolved RNA aptamer.
In: RNA (New York, N.Y.), 24 (4), pp. 480-485. ISSN 1469-9001,
[Article]

Abstract

Inhibitory aptamers against a protein are promising as antagonistic reagents and repressive genetic components. Typically, improvement of such aptamers is achieved by acquiring higher binding affinity. Here, we report an alternative mechanism for the improvement of aptamer activity. Recently, we reported a transcriptional activator based on an inhibitory RNA aptamer against lambda cI repressor. We improved the aptamer through in vitro selection (SELEX) from a randomly mutagenized aptamer pool, followed by in vivo screening and truncation. Biochemical analyses indicated that the activity improvement was achieved by alteration of the complex formation stoichiometry, rather than by higher affinity or expression. Our results suggest an alternative strategy for improving aptamer activity.

Item Type: Article
Erschienen: 2018
Creators: Ohuchi, Shoji ; Suess, Beatrix
Title: Altered stoichiometry of an evolved RNA aptamer.
Language: English
Abstract:

Inhibitory aptamers against a protein are promising as antagonistic reagents and repressive genetic components. Typically, improvement of such aptamers is achieved by acquiring higher binding affinity. Here, we report an alternative mechanism for the improvement of aptamer activity. Recently, we reported a transcriptional activator based on an inhibitory RNA aptamer against lambda cI repressor. We improved the aptamer through in vitro selection (SELEX) from a randomly mutagenized aptamer pool, followed by in vivo screening and truncation. Biochemical analyses indicated that the activity improvement was achieved by alteration of the complex formation stoichiometry, rather than by higher affinity or expression. Our results suggest an alternative strategy for improving aptamer activity.

Journal or Publication Title: RNA (New York, N.Y.)
Journal volume: 24
Number: 4
Divisions: 10 Department of Biology
10 Department of Biology > Synthetic Genetic Circuits
Date Deposited: 03 Jan 2018 10:33
Identification Number: pmid:29284756
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