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An inhibitory RNA aptamer against the lambda cI repressor shows transcriptional activator activity in vivo.

Ohuchi, Shoji ; Suess, Beatrix (2017):
An inhibitory RNA aptamer against the lambda cI repressor shows transcriptional activator activity in vivo.
In: FEBS letters, 591 (10), pp. 1429-1436. ISSN 1873-3468,
[Article]

Abstract

An RNA aptamer is one of the promising components for constructing artificial genetic circuits. In this study, we developed a transcriptional activator based on an RNA aptamer against one of the most frequently applied repressor proteins, lambda phage cI. In vitro selection (SELEX), followed by in vivo screening identified an RNA aptamer with the intended transcriptional activator activity from an RNA pool containing a 40-nucleotide long random region. Quantitative analysis showed 35-fold elevation of reporter expression upon aptamer expression. These results suggest that the diversity of artificial transcriptional activators can be extended by employing RNA aptamers against repressor proteins to broaden the tools available for constructing genetic circuits. This article is protected by copyright. All rights reserved.

Item Type: Article
Erschienen: 2017
Creators: Ohuchi, Shoji ; Suess, Beatrix
Title: An inhibitory RNA aptamer against the lambda cI repressor shows transcriptional activator activity in vivo.
Language: English
Abstract:

An RNA aptamer is one of the promising components for constructing artificial genetic circuits. In this study, we developed a transcriptional activator based on an RNA aptamer against one of the most frequently applied repressor proteins, lambda phage cI. In vitro selection (SELEX), followed by in vivo screening identified an RNA aptamer with the intended transcriptional activator activity from an RNA pool containing a 40-nucleotide long random region. Quantitative analysis showed 35-fold elevation of reporter expression upon aptamer expression. These results suggest that the diversity of artificial transcriptional activators can be extended by employing RNA aptamers against repressor proteins to broaden the tools available for constructing genetic circuits. This article is protected by copyright. All rights reserved.

Journal or Publication Title: FEBS letters
Journal volume: 591
Number: 10
Divisions: 10 Department of Biology
10 Department of Biology > Synthetic Genetic Circuits
Date Deposited: 19 Apr 2017 12:20
Identification Number: pmid:28407231
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