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In vitro selection of antibiotic-binding aptamers.

Groher, Florian ; Suess, Beatrix (2016):
In vitro selection of antibiotic-binding aptamers.
In: Methods (San Diego, Calif.), 106, pp. 42-50. ISSN 1095-9130,
[Article]

Abstract

Despite its wide applicability the selection of small molecule-binding RNA aptamers with high affinity binding and specificity is still challenging. We will present here a protocol which allows the in vitro selection of antibiotic-binding aptamers which turned out to be important building blocks for the design process of synthetic riboswitches. The presented methods will be compared with alternative in vitro selection protocols. A detailed note section will point out useful tips and pitfalls.

Item Type: Article
Erschienen: 2016
Creators: Groher, Florian ; Suess, Beatrix
Title: In vitro selection of antibiotic-binding aptamers.
Language: English
Abstract:

Despite its wide applicability the selection of small molecule-binding RNA aptamers with high affinity binding and specificity is still challenging. We will present here a protocol which allows the in vitro selection of antibiotic-binding aptamers which turned out to be important building blocks for the design process of synthetic riboswitches. The presented methods will be compared with alternative in vitro selection protocols. A detailed note section will point out useful tips and pitfalls.

Journal or Publication Title: Methods (San Diego, Calif.)
Journal volume: 106
Divisions: 10 Department of Biology
10 Department of Biology > Synthetic Genetic Circuits
Date Deposited: 31 May 2016 06:46
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