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A dual control mechanism synchronizes riboflavin and sulphur metabolism in Bacillus subtilis.

Pedrolli, Danielle Biscaro ; Kühm, Christian ; Sévin, Daniel C. ; Vockenhuber, Michael P. ; Sauer, Uwe ; Suess, Beatrix ; Mack, Matthias (2015):
A dual control mechanism synchronizes riboflavin and sulphur metabolism in Bacillus subtilis.
In: Proceedings of the National Academy of Sciences of the United States of America, 112 (45), pp. 14054-14059. ISSN 1091-6490,
[Article]

Abstract

Flavin mononucleotide (FMN) riboswitches are genetic elements, which in many bacteria control genes responsible for biosynthesis and/or transport of riboflavin (rib genes). Cytoplasmic riboflavin is rapidly and almost completely converted to FMN by flavokinases. When cytoplasmic levels of FMN are sufficient ("high levels"), FMN binding to FMN riboswitches leads to a reduction of rib gene expression. We report here that the protein RibR counteracts the FMN-induced "turn-off" activities of both FMN riboswitches in Bacillus subtilis, allowing rib gene expression even in the presence of high levels of FMN. The reason for this secondary metabolic control by RibR is to couple sulfur metabolism with riboflavin metabolism.

Item Type: Article
Erschienen: 2015
Creators: Pedrolli, Danielle Biscaro ; Kühm, Christian ; Sévin, Daniel C. ; Vockenhuber, Michael P. ; Sauer, Uwe ; Suess, Beatrix ; Mack, Matthias
Title: A dual control mechanism synchronizes riboflavin and sulphur metabolism in Bacillus subtilis.
Language: English
Abstract:

Flavin mononucleotide (FMN) riboswitches are genetic elements, which in many bacteria control genes responsible for biosynthesis and/or transport of riboflavin (rib genes). Cytoplasmic riboflavin is rapidly and almost completely converted to FMN by flavokinases. When cytoplasmic levels of FMN are sufficient ("high levels"), FMN binding to FMN riboswitches leads to a reduction of rib gene expression. We report here that the protein RibR counteracts the FMN-induced "turn-off" activities of both FMN riboswitches in Bacillus subtilis, allowing rib gene expression even in the presence of high levels of FMN. The reason for this secondary metabolic control by RibR is to couple sulfur metabolism with riboflavin metabolism.

Journal or Publication Title: Proceedings of the National Academy of Sciences of the United States of America
Journal volume: 112
Number: 45
Divisions: 10 Department of Biology
10 Department of Biology > Synthetic Genetic Circuits
Date Deposited: 09 Nov 2015 08:09
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