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Optogenetics. Engineering of a light-gated potassium channel.

Cosentino, Cristian ; Alberio, Laura ; Gazzarrini, Sabrina ; Aquila, Marco ; Romano, Edoardo ; Cermenati, Solei ; Zuccolini, Paolo ; Petersen, Jan ; Beltrame, Monica ; Etten, James L. van ; Christie, John M. ; Thiel, Gerhard ; Moroni, Anna (2015):
Optogenetics. Engineering of a light-gated potassium channel.
In: Science (New York, N.Y.), 348 (6235), pp. 707-10. ISSN 1095-9203,
[Article]

Abstract

The present palette of opsin-based optogenetic tools lacks a light-gated potassium (K(+)) channel desirable for silencing of excitable cells. Here, we describe the construction of a blue-light-induced K(+) channel 1 (BLINK1) engineered by fusing the plant LOV2-Jα photosensory module to the small viral K(+) channel Kcv. BLINK1 exhibits biophysical features of Kcv, including K(+) selectivity and high single-channel conductance but reversibly photoactivates in blue light. Opening of BLINK1 channels hyperpolarizes the cell to the K(+) equilibrium potential. Ectopic expression of BLINK1 reversibly inhibits the escape response in light-exposed zebrafish larvae. BLINK1 therefore provides a single-component optogenetic tool that can establish prolonged, physiological hyperpolarization of cells at low light intensities.

Item Type: Article
Erschienen: 2015
Creators: Cosentino, Cristian ; Alberio, Laura ; Gazzarrini, Sabrina ; Aquila, Marco ; Romano, Edoardo ; Cermenati, Solei ; Zuccolini, Paolo ; Petersen, Jan ; Beltrame, Monica ; Etten, James L. van ; Christie, John M. ; Thiel, Gerhard ; Moroni, Anna
Title: Optogenetics. Engineering of a light-gated potassium channel.
Language: German
Abstract:

The present palette of opsin-based optogenetic tools lacks a light-gated potassium (K(+)) channel desirable for silencing of excitable cells. Here, we describe the construction of a blue-light-induced K(+) channel 1 (BLINK1) engineered by fusing the plant LOV2-Jα photosensory module to the small viral K(+) channel Kcv. BLINK1 exhibits biophysical features of Kcv, including K(+) selectivity and high single-channel conductance but reversibly photoactivates in blue light. Opening of BLINK1 channels hyperpolarizes the cell to the K(+) equilibrium potential. Ectopic expression of BLINK1 reversibly inhibits the escape response in light-exposed zebrafish larvae. BLINK1 therefore provides a single-component optogenetic tool that can establish prolonged, physiological hyperpolarization of cells at low light intensities.

Journal or Publication Title: Science (New York, N.Y.)
Volume of the journal: 348
Issue Number: 6235
Divisions: 10 Department of Biology
10 Department of Biology > Plant Membrane Biophysics
Date Deposited: 29 Jun 2015 12:44
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