Klein, Katharina ; Gigler, Alexander M. ; Aschenbrenner, Thomas ; Monetti, Roberto ; Bunk, Wolfram ; Jamitzky, Ferdinand ; Morfill, Gregor ; Stark, Robert W. ; Schlegel, Jürgen (2012)
Label-Free Live-Cell Imaging with Confocal Raman Microscopy.
In: Biophysical Journal, 102 (2)
doi: 10.1016/j.bpj.2011.12.027
Article, Bibliographie
Abstract
Confocal Raman spectroscopy is a noninvasive alternative to established cell imaging methods because it does not require chemical fixation, the use of fluorescent markers, or genetic engineering. In particular, single live-cell, high-resolution imaging by confocal Raman microscopy is desirable because it allows further experiments concerning the individually investigated cells. However, to derive meaningful images from the spectroscopic data, one must identify cell components within the dataset. Using immunofluorescence images as a reference, we derive Raman spectral signatures by means of information measures to identify cell components such as the nucleus, the endoplasmic reticulum, the Golgi apparatus, and mitochondria. The extracted signatures allow us to generate representations equivalent to conventional (immuno)fluorescence images with more than three cell components at a time, exploiting the Raman spectral information alone.
| Item Type: | Article |
|---|---|
| Erschienen: | 2012 |
| Creators: | Klein, Katharina ; Gigler, Alexander M. ; Aschenbrenner, Thomas ; Monetti, Roberto ; Bunk, Wolfram ; Jamitzky, Ferdinand ; Morfill, Gregor ; Stark, Robert W. ; Schlegel, Jürgen |
| Type of entry: | Bibliographie |
| Title: | Label-Free Live-Cell Imaging with Confocal Raman Microscopy |
| Language: | English |
| Date: | 18 January 2012 |
| Publisher: | Elsevier Science Publishing |
| Journal or Publication Title: | Biophysical Journal |
| Volume of the journal: | 102 |
| Issue Number: | 2 |
| DOI: | 10.1016/j.bpj.2011.12.027 |
| Abstract: | Confocal Raman spectroscopy is a noninvasive alternative to established cell imaging methods because it does not require chemical fixation, the use of fluorescent markers, or genetic engineering. In particular, single live-cell, high-resolution imaging by confocal Raman microscopy is desirable because it allows further experiments concerning the individually investigated cells. However, to derive meaningful images from the spectroscopic data, one must identify cell components within the dataset. Using immunofluorescence images as a reference, we derive Raman spectral signatures by means of information measures to identify cell components such as the nucleus, the endoplasmic reticulum, the Golgi apparatus, and mitochondria. The extracted signatures allow us to generate representations equivalent to conventional (immuno)fluorescence images with more than three cell components at a time, exploiting the Raman spectral information alone. |
| Divisions: | 11 Department of Materials and Earth Sciences 11 Department of Materials and Earth Sciences > Material Science 11 Department of Materials and Earth Sciences > Material Science > Physics of Surfaces DFG-Collaborative Research Centres (incl. Transregio) DFG-Collaborative Research Centres (incl. Transregio) > Collaborative Research Centres Exzellenzinitiative Exzellenzinitiative > Clusters of Excellence Zentrale Einrichtungen Exzellenzinitiative > Clusters of Excellence > Center of Smart Interfaces (CSI) |
| Date Deposited: | 16 Jun 2014 09:31 |
| Last Modified: | 21 Mar 2019 11:55 |
| PPN: | |
| Funders: | This work was supported by the Deutsche Forschungsgemeinschaft Cluster of Excellence “Nanosystems Initiative Munich” and grant DFG-SFB-824 (Project B6: Imaging for Selection, Monitoring and Individualization of Cancer Therapies). |
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