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Monitoring the expression level of coding and non-coding RNAs using a TetR inducing aptamer tag.

Meitert, Johannes and Aram, Ronny and Wiesemann, Katharina and Weigand, Julia E. and Suess, Beatrix (2013):
Monitoring the expression level of coding and non-coding RNAs using a TetR inducing aptamer tag.
In: Bioorganic & medicinal chemistry, 21 (20), pp. 6233-8. ISSN 1464-3391,
[Article]

Abstract

RNA aptamers have been widely used as regulators for conditional gene expression. The TetR binding aptamer can activate tetracycline repressor TetR controlled gene expression with high efficiency. Here we demonstrate that the aptamer can also activate TetR controlled gene expression when expressed in the context of a natural transcripts. The aptamer was inserted into the untranslated regions of mRNAs as well as into small non-coding RNAs and was expressed both from a plasmid and from an endogenous locus. Our data suggest that the aptamer is a valuable tool to easily monitor the expression level of different RNAs, and it therefore represents a powerful tool for the construction of complex synthetic gene networks.

Item Type: Article
Erschienen: 2013
Creators: Meitert, Johannes and Aram, Ronny and Wiesemann, Katharina and Weigand, Julia E. and Suess, Beatrix
Title: Monitoring the expression level of coding and non-coding RNAs using a TetR inducing aptamer tag.
Language: English
Abstract:

RNA aptamers have been widely used as regulators for conditional gene expression. The TetR binding aptamer can activate tetracycline repressor TetR controlled gene expression with high efficiency. Here we demonstrate that the aptamer can also activate TetR controlled gene expression when expressed in the context of a natural transcripts. The aptamer was inserted into the untranslated regions of mRNAs as well as into small non-coding RNAs and was expressed both from a plasmid and from an endogenous locus. Our data suggest that the aptamer is a valuable tool to easily monitor the expression level of different RNAs, and it therefore represents a powerful tool for the construction of complex synthetic gene networks.

Journal or Publication Title: Bioorganic & medicinal chemistry
Journal volume: 21
Number: 20
Divisions: 10 Department of Biology
10 Department of Biology > Synthetic Genetic Circuits
10 Department of Biology > RNA Biochemistry
Date Deposited: 29 Oct 2013 14:39
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