Heinrich, Eva-Marie ; Wagner, Jasmin ; Krüger, Marcus ; John, David ; Uchida, Shizuka ; Weigand, Julia E. ; Suess, Beatrix ; Dimmeler, Stefanie (2013)
Regulation of miR-17-92a cluster processing by the microRNA binding protein SND1.
In: FEBS letters, 587 (15)
Article, Bibliographie
Abstract
MicroRNAs are small non-coding RNAs that regulate gene expression. Although all seven members of the miR-17-92a cluster originate from one primary transcript they are differentially expressed suggesting the presence of posttranscriptional regulation. By RNA pulldown and mass spectrometry we identified SND1, a known regulator of edited RNAs, interacting with pre-miR-92a and all mature miR-17-92a members. Hypoxic conditions lead to an elevation of the pri-miR-17-92a transcript and significantly increased levels of the precursors whereas the mature miRs were not significantly changed. SND1 silencing resolved this block in processing and induced an increase in mature miRs. Together, SND1 might be the missing link between hypoxia and the differential regulation of miRNA processing.
| Item Type: | Article |
|---|---|
| Erschienen: | 2013 |
| Creators: | Heinrich, Eva-Marie ; Wagner, Jasmin ; Krüger, Marcus ; John, David ; Uchida, Shizuka ; Weigand, Julia E. ; Suess, Beatrix ; Dimmeler, Stefanie |
| Type of entry: | Bibliographie |
| Title: | Regulation of miR-17-92a cluster processing by the microRNA binding protein SND1. |
| Language: | English |
| Date: | 2013 |
| Journal or Publication Title: | FEBS letters |
| Volume of the journal: | 587 |
| Issue Number: | 15 |
| Abstract: | MicroRNAs are small non-coding RNAs that regulate gene expression. Although all seven members of the miR-17-92a cluster originate from one primary transcript they are differentially expressed suggesting the presence of posttranscriptional regulation. By RNA pulldown and mass spectrometry we identified SND1, a known regulator of edited RNAs, interacting with pre-miR-92a and all mature miR-17-92a members. Hypoxic conditions lead to an elevation of the pri-miR-17-92a transcript and significantly increased levels of the precursors whereas the mature miRs were not significantly changed. SND1 silencing resolved this block in processing and induced an increase in mature miRs. Together, SND1 might be the missing link between hypoxia and the differential regulation of miRNA processing. |
| Divisions: | 10 Department of Biology 10 Department of Biology > Synthetic Genetic Circuits (2020 renamed "Synthetic RNA biology) 10 Department of Biology > RNA Biochemistry |
| Date Deposited: | 03 Sep 2013 11:24 |
| Last Modified: | 06 Nov 2020 07:15 |
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