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Aptamers as artificial gene regulation elements.

Suess, Beatrix and Weigand, Julia E. (2009):
Aptamers as artificial gene regulation elements.
In: Methods in molecular biology (Clifton, N.J.), 535, pp. 201-8. ISSN 1064-3745,
[Article]

Abstract

Conditional gene expression systems are important tools to identify the function of essential genes or in terms of gene therapy approaches. Small molecule-binding aptamers can be used for efficient control of gene expression by inserting them into the 5' untranslated region of an mRNA with the ligand-bound form inhibiting gene expression by interfering with translation initiation. However, only a small fraction of in vitro selected aptamers has the potential to act as regulator of gene expression which originates the necessity to develop screening systems for the identification of regulatory active aptamers. We describe here a simple and powerful yeast-based screening system which allows the rapid identification of small molecule-binding aptamers with the potential to act as artificial riboswitches for conditional control of gene expression.

Item Type: Article
Erschienen: 2009
Creators: Suess, Beatrix and Weigand, Julia E.
Title: Aptamers as artificial gene regulation elements.
Language: English
Abstract:

Conditional gene expression systems are important tools to identify the function of essential genes or in terms of gene therapy approaches. Small molecule-binding aptamers can be used for efficient control of gene expression by inserting them into the 5' untranslated region of an mRNA with the ligand-bound form inhibiting gene expression by interfering with translation initiation. However, only a small fraction of in vitro selected aptamers has the potential to act as regulator of gene expression which originates the necessity to develop screening systems for the identification of regulatory active aptamers. We describe here a simple and powerful yeast-based screening system which allows the rapid identification of small molecule-binding aptamers with the potential to act as artificial riboswitches for conditional control of gene expression.

Journal or Publication Title: Methods in molecular biology (Clifton, N.J.)
Journal volume: 535
Divisions: 10 Department of Biology
10 Department of Biology > Synthetic Genetic Circuits
10 Department of Biology > RNA Biochemistry
Date Deposited: 22 Feb 2012 10:28
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