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PELDOR spectroscopy reveals preorganization of the neomycin-responsive riboswitch tertiary structure.

Krstić, Ivan and Frolow, Olga and Sezer, Deniz and Endeward, Burkhard and Weigand, Julia E. and Suess, Beatrix and Engels, Joachim W. and Prisner, Thomas F. (2010):
PELDOR spectroscopy reveals preorganization of the neomycin-responsive riboswitch tertiary structure.
In: Journal of the American Chemical Society, 132 (5), pp. 1454-5. ISSN 1520-5126,
[Article]

Abstract

Pulsed electron double resonance (PELDOR) spectroscopy reveals a prearranged tertiary structure of the 27 nucleotides long engineered neomycin-responsive riboswitch. Measured distances between spin labels at positions U4-U14, U4-U15, U14-U26, and U15-U26 were unchanged upon neomycin binding which implies that the global stem-loop architecture is preserved in the absence and presence of the ligand. On the basis of our results, we infer that low-temperature PELDOR data unambiguously demonstrate the existence of an enthalpically favorable set of RNA conformations ready to bind the ligand without major global rearrangement.

Item Type: Article
Erschienen: 2010
Creators: Krstić, Ivan and Frolow, Olga and Sezer, Deniz and Endeward, Burkhard and Weigand, Julia E. and Suess, Beatrix and Engels, Joachim W. and Prisner, Thomas F.
Title: PELDOR spectroscopy reveals preorganization of the neomycin-responsive riboswitch tertiary structure.
Language: English
Abstract:

Pulsed electron double resonance (PELDOR) spectroscopy reveals a prearranged tertiary structure of the 27 nucleotides long engineered neomycin-responsive riboswitch. Measured distances between spin labels at positions U4-U14, U4-U15, U14-U26, and U15-U26 were unchanged upon neomycin binding which implies that the global stem-loop architecture is preserved in the absence and presence of the ligand. On the basis of our results, we infer that low-temperature PELDOR data unambiguously demonstrate the existence of an enthalpically favorable set of RNA conformations ready to bind the ligand without major global rearrangement.

Journal or Publication Title: Journal of the American Chemical Society
Journal volume: 132
Number: 5
Divisions: 10 Department of Biology
10 Department of Biology > Synthetic Genetic Circuits
10 Department of Biology > RNA Biochemistry
Date Deposited: 22 Feb 2012 10:25
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