Schmidtke, Sina R. ; Duchardt-Ferner, Elke ; Weigand, Julia E. ; Suess, Beatrix ; Wöhnert, Jens (2010)
NMR resonance assignments of an engineered neomycin-sensing riboswitch RNA bound to ribostamycin and tobramycin.
In: Biomolecular NMR assignments, 4 (1)
Article, Bibliographie
Abstract
The neomycin-sensing riboswitch is an engineered riboswitch developed to regulate gene expression in vivo in the lower eukaryote Saccharomyces cerevisiae upon binding to neomycin B. With a size of only 27nt it is the smallest functional riboswitch element identified so far. It binds not only neomycin B but also related aminoglycosides of the 2'-deoxystreptamine class with high affinity. The regulatory activity, however, strongly depends on the identity of the aminoglycoside. As a prerequisite for the structure determination of riboswitch-ligand complexes we report here the (1)H, (15)N, (13)C and partial (31)P chemical shift assignments for the minimal functional 27nt neomycin sensing riboswitch RNA in complex with the 4,5-linked neomycin analog ribostamycin and the 4,6-linked aminoglycoside tobramycin.
| Item Type: | Article |
|---|---|
| Erschienen: | 2010 |
| Creators: | Schmidtke, Sina R. ; Duchardt-Ferner, Elke ; Weigand, Julia E. ; Suess, Beatrix ; Wöhnert, Jens |
| Type of entry: | Bibliographie |
| Title: | NMR resonance assignments of an engineered neomycin-sensing riboswitch RNA bound to ribostamycin and tobramycin. |
| Language: | English |
| Date: | 2010 |
| Journal or Publication Title: | Biomolecular NMR assignments |
| Volume of the journal: | 4 |
| Issue Number: | 1 |
| Abstract: | The neomycin-sensing riboswitch is an engineered riboswitch developed to regulate gene expression in vivo in the lower eukaryote Saccharomyces cerevisiae upon binding to neomycin B. With a size of only 27nt it is the smallest functional riboswitch element identified so far. It binds not only neomycin B but also related aminoglycosides of the 2'-deoxystreptamine class with high affinity. The regulatory activity, however, strongly depends on the identity of the aminoglycoside. As a prerequisite for the structure determination of riboswitch-ligand complexes we report here the (1)H, (15)N, (13)C and partial (31)P chemical shift assignments for the minimal functional 27nt neomycin sensing riboswitch RNA in complex with the 4,5-linked neomycin analog ribostamycin and the 4,6-linked aminoglycoside tobramycin. |
| Divisions: | 10 Department of Biology 10 Department of Biology > Synthetic Genetic Circuits (2020 renamed "Synthetic RNA biology) 10 Department of Biology > RNA Biochemistry |
| Date Deposited: | 22 Feb 2012 10:24 |
| Last Modified: | 06 Nov 2020 07:19 |
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