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A ribosomal ambiguity mutation in the 530 loop of E. coli 16S rRNA.

O'Connor, M. ; Göringer, H. Ulrich ; Dahlberg, A. E. (1992):
A ribosomal ambiguity mutation in the 530 loop of E. coli 16S rRNA.
In: Nucleic acids research, 20 (16), pp. 4221-7. ISSN 0305-1048,
[Article]

Abstract

A series of base substitution and deletion mutations were constructed in the highly conserved 530 stem and loop region of E. coli 16S rRNA involved in binding of tRNA to the ribosomal A site. Base substitution and deletion of G517 produced significant effects on cell growth rate and translational fidelity, permitting readthrough of UGA, UAG and UAA stop codons as well as stimulating +1 and -1 frameshifting in vivo. By contrast, mutations at position 534 had little or no effect on growth rate or translational fidelity. The results demonstrate the importance of G517 in maintaining translational fidelity but do not support a base pairing interaction between G517 and U534.

Item Type: Article
Erschienen: 1992
Creators: O'Connor, M. ; Göringer, H. Ulrich ; Dahlberg, A. E.
Title: A ribosomal ambiguity mutation in the 530 loop of E. coli 16S rRNA.
Language: English
Abstract:

A series of base substitution and deletion mutations were constructed in the highly conserved 530 stem and loop region of E. coli 16S rRNA involved in binding of tRNA to the ribosomal A site. Base substitution and deletion of G517 produced significant effects on cell growth rate and translational fidelity, permitting readthrough of UGA, UAG and UAA stop codons as well as stimulating +1 and -1 frameshifting in vivo. By contrast, mutations at position 534 had little or no effect on growth rate or translational fidelity. The results demonstrate the importance of G517 in maintaining translational fidelity but do not support a base pairing interaction between G517 and U534.

Journal or Publication Title: Nucleic acids research
Volume of the journal: 20
Issue Number: 16
Divisions: 10 Department of Biology > Postranscriptional Gene Regulation and RNA Therapeutics
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10 Department of Biology
Date Deposited: 03 Nov 2011 13:50
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